Characterization of hydrogen sulfide and its synthases, cystathionine β-synthase and cystathionine γ-lyase, in human prostatic tissue and cells.

Abstract

OBJECTIVE:

To investigate hydrogen sulfide and its synthases, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE), in human prostatic tissue and cells.

METHODS:

CBS and CSE in human prostatic tissue and cells were located using immunostaining. Western blot and a sulfur-sensitive electrode were used to evaluate the expression levels and catalytic activity of CBS and CSE. We analyzed the association between dihydrotestosterone-added or hormone-reduced medium-induced CBS/CSE protein levels with androgen receptor levels in prostate cancer lines. All experiments were repeated ≥3 times.

RESULTS:

Endogenous hydrogen sulfide and its synthases existed in various areas of human prostatic tissue and cells. Cell activity and CBS/CSE protein levels were greatest in the androgen-dependent prostate cancer cell LNCaP among all cells and downregulated by dihydrotestosterone.

CONCLUSION:

Hydrogen sulfide and its synthases in human prostatic tissue and cells were modulated by dihydrotestosterone, which could suggest a potential therapy for prostatic disease.

• Authors: Hui Guo, Jun-Wei Gai, Ying Wang, Hong-Fang Jin, Jun-Bao Du, Jie Jin
• Journal: Urology (Urology) Vol. 79 Issue 2 Pg. 483.e1-5 (Feb 2012) ISSN: 1527-9995 [Electronic] United States
• PMID: 22310774 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Androgens
• Culture Media
• Neoplasm Proteins
• Receptors, Androgen
• Dihydrotestosterone
• Cystathionine beta-Synthase
• Cystathionine gamma-Lyase
• Hydrogen Sulfide

Topics

• Adenocarcinoma (chemistry, enzymology, pathology)
• Androgens
• Cell Line (chemistry, drug effects, enzymology)
• Cell Line, Tumor (chemistry, drug effects, enzymology)
• Culture Media (pharmacology)
• Cystathionine beta-Synthase (analysis, biosynthesis, genetics)
• Cystathionine gamma-Lyase (analysis, biosynthesis, genetics)
• Dihydrotestosterone (pharmacology)
• Down-Regulation (drug effects)
• Enzyme Induction (drug effects)
• Epithelial Cells (chemistry, drug effects, enzymology)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Hydrogen Sulfide (analysis)
• Male
• Neoplasm Proteins (analysis, biosynthesis, genetics)
• Neoplasms, Hormone-Dependent (chemistry, enzymology, pathology)
• Prostate (chemistry, cytology, enzymology)
• Prostatic Neoplasms (chemistry, enzymology, pathology)
• Receptors, Androgen (analysis, biosynthesis, genetics)
• Stromal Cells (chemistry, drug effects, enzymology)