Proteomic profiling of follicular and papillary thyroid tumors.

Abstract

OBJECTIVE:

Thyroid proteomics is a new direction in thyroid cancer research aiming at etiological understanding and biomarker identification for improved diagnosis.

METHODS:

Two-dimensional electrophoresis was applied to cytosolic protein extracts from frozen thyroid samples (ten follicular adenomas, nine follicular carcinomas, ten papillary carcinomas, and ten reference thyroids). Spots with differential expression were revealed by image and multivariate statistical analyses, and identified by mass spectrometry.

RESULTS:

A set of 25 protein spots significant for discriminating between the sample groups was identified. Proteins identified for nine of these spots were studied further including 14-3-3 protein beta/alpha, epsilon, and zeta/delta, peroxiredoxin 6, selenium-binding protein 1, protein disulfide-isomerase precursor, annexin A5 (ANXA5), tubulin alpha-1B chain, and α1-antitrypsin precursor. This subset of protein spots carried the same predictive power in differentiating between follicular carcinoma and adenoma or between follicular and papillary carcinoma, as compared with the larger set of 25 spots. Protein expression in the sample groups was demonstrated by western blot analyses. For ANXA5 and the 14-3-3 proteins, expression in tumor cell cytoplasm was demonstrated by immunohistochemistry both in the sample groups and an independent series of papillary thyroid carcinomas.

CONCLUSION:

The proteins identified confirm previous findings in thyroid proteomics, and suggest additional proteins as dysregulated in thyroid tumors.

• Authors: Anastasios Sofiadis, Susanne Becker, Ulf Hellman, Lina Hultin-Rosenberg, Andrii Dinets, Mykola Hulchiy, Jan Zedenius, Göran Wallin, Theodoros Foukakis, Anders Höög, Gert Auer, Janne Lehtiö, Catharina Larsson
• Journal: European journal of endocrinology (Eur J Endocrinol) Vol. 166 Issue 4 Pg. 657-67 (Apr 2012) ISSN: 1479-683X [Electronic] England
• PMID: 22275472 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor

Topics

• Adenocarcinoma, Follicular
• Biomarkers, Tumor (analysis, metabolism)
• Carcinoma
• Carcinoma, Papillary
• Diagnosis, Differential
• Electrophoresis, Gel, Two-Dimensional (methods)
• Humans
• Immunohistochemistry
• Mass Spectrometry
• Predictive Value of Tests
• Protein Array Analysis (methods)
• Proteomics (methods)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Thyroid Cancer, Papillary
• Thyroid Neoplasms (diagnosis, metabolism, pathology)
• Validation Studies as Topic