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Epigenetically mediated downregulation of the differentiation-promoting chaperon protein CRABP2 in astrocytic gliomas.

Abstract
Impairment of endogenous differentiation pathways like retinoic acid (RA) signaling seems to be a central pathogenetic event in astrocytic gliomas. Among others, expression of the differentiation-promoting RA chaperon protein cellular retinoic acid binding protein 2 (CRABP2) is extenuated in high-grade gliomas. Against this background, we aimed at identifying potential pathomechanisms underlying reduced CRABP2 expression in these tumors. Using MassARRAY methylation analysis, we detected extensive CpG methylation upstream of the CRABP2 gene locus in a study sample comprising 100 astrocytic gliomas of WHO Grade II to IV. Compared to nontumorous control samples, tumors revealed increased CpG methylation and methylation levels were inversely correlated to CRABP2 mRNA expression. Substantiating our in situ findings, CRABP2 mRNA levels increased in glioma cell lines after exposure to the demethylating agent 5-aza-2'-deoxycytidine. Finally, a distinct CpG methylation signature distinguished between primary glioblastoma on the one hand and the group of astrocytoma WHO II-III and secondary glioblastoma on the other hand. Altogether, our observations suggest that epigenetic silencing of CRABP2 might contribute to an immature phenotype in glioma cells.
AuthorsBenito Campos, Rolf Warta, Jittiporn Chaisaingmongkol, Lea Geiselhart, Odilia Popanda, Christian Hartmann, Andreas von Deimling, Andreas Unterberg, Christoph Plass, Peter Schmezer, Christel Herold-Mende
JournalInternational journal of cancer (Int J Cancer) Vol. 131 Issue 8 Pg. 1963-8 (Oct 15 2012) ISSN: 1097-0215 [Electronic] United States
PMID22275178 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 UICC.
Chemical References
  • Receptors, Retinoic Acid
  • retinoic acid binding protein II, cellular
  • IDH2 protein, human
  • Isocitrate Dehydrogenase
  • IDH1 protein, human
Topics
  • Astrocytoma (genetics)
  • Brain (metabolism)
  • Brain Neoplasms (genetics)
  • Case-Control Studies
  • Cell Differentiation
  • CpG Islands
  • DNA Methylation
  • Epigenesis, Genetic
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Isocitrate Dehydrogenase (genetics)
  • Neoplasm Grading
  • Receptors, Retinoic Acid (genetics)
  • Signal Transduction

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