The effects of running exercise on oxidative capacity and PGC-1α mRNA levels in the soleus muscle of rats with metabolic syndrome.

Abstract

Skeletal muscles in animals with metabolic syndrome exhibit reduced oxidative capacity. We investigated the effects of running exercise on fiber characteristics, oxidative capacity, and mRNA levels in the soleus muscles of rats with metabolic syndrome [SHR/NDmcr-cp (cp/cp); CP]. We divided 5-week-old CP rats into non-exercise (CP) and exercise (CP-Ex) groups. Wistar-Kyoto rats (WKY) were used as the control group. CP-Ex rats were permitted voluntary exercise on running wheels for 10 weeks. Triglyceride levels were higher and adiponectin levels lower in the CP and CP-Ex groups than in the WKY group. However, triglyceride levels were lower and adiponectin levels higher in the CP-Ex group than in the CP group. The soleus muscles in CP-Ex rats contained only high-oxidative type I fibers, whereas those in WKY and CP rats contained type I, IIA, and IIC fibers. Muscle succinate dehydrogenase (SDH) activity was higher in the CP-Ex group than in the CP group; there was no difference in SDH activity between the WKY and CP-Ex groups. Muscle proliferator-activated receptor γ coactivator-1α (PGC-1α) mRNA levels were higher in the CP-Ex group than in the CP group; there was no difference in PGC-1α mRNA levels between the WKY and CP-Ex groups. In CP-Ex rats, longer running distance was associated with increased muscle SDH activity and PGC-1α mRNA levels. We concluded that running exercise restored decreased muscle oxidative capacity and PGC-1α mRNA levels and improved hypertriglyceridemia in rats with metabolic syndrome.

• Authors: Fumiko Nagatomo, Hidemi Fujino, Hiroyo Kondo, Motoki Kouzaki, Ning Gu, Isao Takeda, Kinsuke Tsuda, Akihiko Ishihara
• Journal: The journal of physiological sciences : JPS (J Physiol Sci) Vol. 62 Issue 2 Pg. 105-14 (Mar 2012) ISSN: 1880-6562 [Electronic] Japan
• PMID: 22234788 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• DNA Primers
• Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
• Ppargc1a protein, rat
• RNA, Messenger
• RNA-Binding Proteins
• Transcription Factors
• Succinate Dehydrogenase

Topics

• Animals
• Base Sequence
• Blood Glucose (metabolism)
• DNA Primers (genetics)
• Disease Models, Animal
• Hypertriglyceridemia (blood, physiopathology, therapy)
• Male
• Metabolic Syndrome (blood, genetics, physiopathology, therapy)
• Muscle Fibers, Skeletal (metabolism)
• Muscle, Skeletal (metabolism)
• Oxidation-Reduction
• Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
• Physical Exertion (physiology)
• RNA, Messenger (genetics, metabolism)
• RNA-Binding Proteins (genetics)
• Rats
• Rats, Inbred WKY
• Rats, Mutant Strains
• Running (physiology)
• Succinate Dehydrogenase (genetics)
• Transcription Factors (genetics)