Serum indoleamine 2,3-dioxygenase activity predicts prognosis of pulmonary tuberculosis.

Abstract

Tuberculosis (TB) continues to be a major health problem, and there are few biomarkers for predicting prognosis. Indoleamine 2,3-dioxygenase (IDO), a potent immunoregulatory molecule, catalyzes the rate-limiting step of tryptophan (Trp) degradation in the kynurenine (Kyn) pathway. An increase in IDO activity determined by the serum Trp/Kyn ratio has been shown to be associated with poor prognosis in cancers and bacteremia. In TB, however, there are no studies measuring serum IDO activity to determine its clinical significance. We evaluated serum IDO activity with 174 pulmonary TB (PTB) patients and 85 controls, using liquid chromatography/electrospray ionization tandem mass spectrometry. IDO activity was estimated by calculating the serum Kyn-to-Trp ratio. PTB patients had significantly higher Kyn concentrations and IDO activity and significantly lower Trp concentrations (P < 0.0001, P < 0.0001, and P < 0.0001, respectively) than the controls. Of 174 PTB patients, 39 (22.4%) died. The patients who died had significantly higher concentrations of Kyn and significantly lower Trp concentrations, resulting in significantly higher IDO activity (P < 0.0001, P < 0.0001, and P < 0.0001, respectively). In a receiver operating characteristic (ROC) analysis, serum IDO activity had the highest area under the curve (0.850), and this activity was an independent prognostic factor in multivariate analysis. These results suggest that serum IDO activity can be used as a novel prognostic marker in PTB.

• Authors: Yuzo Suzuki, Takafumi Suda, Kazuhiro Asada, Seiichi Miwa, Masako Suzuki, Michio Fujie, Kazuki Furuhashi, Yutaro Nakamura, Naoki Inui, Toshihiro Shirai, Hiroshi Hayakawa, Hirotoshi Nakamura, Kingo Chida
• Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 19 Issue 3 Pg. 436-42 (Mar 2012) ISSN: 1556-679X [Electronic] United States
• PMID: 22219312 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Biomarkers
• Indoleamine-Pyrrole 2,3,-Dioxygenase
• Kynurenine
• Tryptophan

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers (blood)
• Chromatography, Liquid
• Female
• Humans
• Indoleamine-Pyrrole 2,3,-Dioxygenase (blood)
• Kynurenine (analysis)
• Male
• Middle Aged
• Prognosis
• Prospective Studies
• ROC Curve
• Serum (enzymology)
• Spectrometry, Mass, Electrospray Ionization
• Tandem Mass Spectrometry
• Tryptophan (analysis)
• Tuberculosis, Pulmonary (diagnosis, pathology)