Abstract | BACKGROUND: METHODS: We measured S100A9 levels in serum and bronchoalveolar lavage fluid (BALF) from 28 patients with IPF, 15 with I-NSIP, 20 with cryptogenic organizing pneumonia (COP), 35 with CVD-IP and 23 healthy individuals (controls) using enzyme-linked immunosorbent assays. S100A9 in the lung was also immunohistochemically localized. RESULTS: S100A9 levels in BALF, but not in serum, were significantly elevated in patients with IPF compared with I-NSIP, COP, CVD-IP and healthy individuals. S100A9 immunoreactivity was localized mainly in macrophages and neutrophils in lung specimens from patients with IPF. The results of receiver operating characteristic (ROC) curve analysis showed that BALF S100A9 levels had sufficient specificity and sensitivity to distinguish IPF from I-NSIP and CVD-IP. CONCLUSION:
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Authors | Atsuko Hara, Noriho Sakamoto, Yuji Ishimatsu, Tomoyuki Kakugawa, Shota Nakashima, Shintaro Hara, Misato Adachi, Hanako Fujita, Hiroshi Mukae, Shigeru Kohno |
Journal | Respiratory medicine
(Respir Med)
Vol. 106
Issue 4
Pg. 571-80
(Apr 2012)
ISSN: 1532-3064 [Electronic] England |
PMID | 22209187
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- Aged
- Biomarkers
(analysis, blood)
- Bronchoalveolar Lavage
(methods)
- Bronchoalveolar Lavage Fluid
(chemistry, cytology)
- Calgranulin B
(analysis, blood)
- Diagnosis, Differential
- Electrophoresis, Gel, Two-Dimensional
(methods)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Humans
- Idiopathic Interstitial Pneumonias
(diagnosis)
- Idiopathic Pulmonary Fibrosis
(diagnosis, pathology)
- Leukocyte Count
- Lung Diseases, Interstitial
(diagnosis)
- Male
- Middle Aged
- Neutrophils
(pathology)
- Prognosis
- Proteomics
(methods)
- Sensitivity and Specificity
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