Abstract | BACKGROUND: METHODS: Ascending thoracic aortic aneurysm tissue from 46 patients with BAVs was examined for MMP/TIMP abundance, and global MMP activity was compared with normal aortic specimens (n=15). Proteolytic balance was calculated as the ratio of MMP abundance to a composite TIMP score. Results were stratified by valve morphologic group (L-N [n=6], R-L [n=31], and R-N [n=9]). RESULTS: The BAV specimens (p<0.05 versus normal aorta, 100%) displayed elevated global MMP activity (273%±63%), MMP-9 (263%±47%), and decreased MMP-7 (56%±10%), MMP-8 (58%±11%), TIMP-1 (63%±7%), and TIMP-4 (38%±3%). The R-L group showed increased global MMP activity (286%±89%) and MMP-9 (267%±55%) with reduced MMP-7 (45%±7%), MMP-8 (68%±15%), TIMP-1 (58%±7%), and TIMP-4 (35%±3%). The L-N group showed elevated global MMP activity (284%±71%) and decreased MMP-8 (37%±17%) and TIMP-4 (48%±14) activity. In the R-N group, MMP-7 (46%±13%) and MMP-8 (36%±17%) and TIMP-1 (59%±10%) and TIMP-4 (42%±5%) were decreased. The R-L group demonstrated an increased proteolytic balance for MMP-1, MMP-9, and MMP-12 relative to L-N and R-N. CONCLUSIONS: Each BAV morphologic group possesses a unique signature of MMPs and TIMPs. MMP/TIMP score ratios suggest that the R-L group may be more aggressive, justifying earlier surgical intervention.
|
Authors | John S Ikonomidis, Jean Marie Ruddy, Stewart M Benton Jr, Jazmine Arroyo, Theresa A Brinsa, Robert E Stroud, Ahmed Zeeshan, Joseph E Bavaria, Joseph H Gorman 3rd, Robert C Gorman, Francis G Spinale, Jeffrey A Jones |
Journal | The Annals of thoracic surgery
(Ann Thorac Surg)
Vol. 93
Issue 2
Pg. 457-63
(Feb 2012)
ISSN: 1552-6259 [Electronic] Netherlands |
PMID | 22206960
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
|
Copyright | Copyright © 2012 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Extracellular Matrix Proteins
- Tissue Inhibitor of Metalloproteinases
- Matrix Metalloproteinases
|
Topics |
- Adult
- Aged
- Aorta
(abnormalities, enzymology, pathology, surgery)
- Aortic Aneurysm, Thoracic
(epidemiology, etiology)
- Aortic Valve
(abnormalities, enzymology, pathology, surgery)
- Blood Vessel Prosthesis Implantation
- Dilatation, Pathologic
(enzymology)
- Disease Susceptibility
- Elastic Tissue
(pathology)
- Extracellular Matrix Proteins
(analysis)
- Female
- Humans
- Male
- Matrix Metalloproteinases
(analysis)
- Middle Aged
- Tissue Inhibitor of Metalloproteinases
(analysis)
|