BLCA-4 expression is related to MMP-9, VEGF, IL-1α and IL-8 in bladder cancer but not to PEDF, TNF-α or angiogenesis.

Abstract

AIM:

BLCA-4 is a specific nuclear matrix protein found in bladder cancer and there is a dearth of study on functional analysis upon this factor. We aimed to discover whether BLCA-4 is related to angiogenesis in bladder cancer.

METHODS:

Fifty-three bladder cancer samples were included for immunohistochemical staining of BLCA-4, matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEGF), interleukin-1α (IL-1α), IL-8, pigment epithelium-derived factor (PEDF), tumour necrosis factor-α (TNF-α) and von Willebrand factor (vWF) for microvessel density (MVD). Expressional levels were scored and grouped by clinicopathological parametres for statistical analysis for correlations.

RESULTS:

Positive correlations were identified between expression of BLCA-4 and IL-1α (p=0.038), IL-8 (p=0.001), VEGF (p=0.002), and MMP-9 (p=0.013). No correlation was found for PEDF (p=0.182), TNF-α (p=0.531) or MVD (p=0.932). Positive correlations were also obtained in cases of advanced grade or stage, larger, recurrent and multiple tumours. Positive correlation between BLCA-4 and MMP-9 was also found in papillary urothelial neoplasm of low malignant potential (PUNLMP).

CONCLUSION:

BLCA-4 may not effect pro-angiogenic pathways in bladder cancer, it can however interact with IL-1α, IL-8, VEGF and MMP-9 to enhance tumourigenesis and tumour invasiveness.

• Authors: C Feng, Z Wu, T Guo, H Jiang, M Guan, Y Zhang, H Wen, Q Ding
• Journal: Pathologie-biologie (Pathol Biol (Paris)) Vol. 60 Issue 3 Pg. e36-40 (Jun 2012) ISSN: 1768-3114 [Electronic] France
• PMID: 22197408 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Masson SAS. All rights reserved.

Chemical References

• BLCA-4 protein, human
• Biomarkers, Tumor
• Eye Proteins
• Interleukin-1alpha
• Interleukin-8
• Nerve Growth Factors
• Nuclear Proteins
• Serpins
• Tumor Necrosis Factor-alpha
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• pigment epithelium-derived factor
• Matrix Metalloproteinase 9

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (metabolism, physiology)
• Carcinoma, Squamous Cell (metabolism, pathology)
• Eye Proteins (metabolism)
• Female
• Humans
• Immunohistochemistry
• Interleukin-1alpha (metabolism, physiology)
• Interleukin-8 (metabolism, physiology)
• Male
• Matrix Metalloproteinase 9 (metabolism, physiology)
• Middle Aged
• Neoplasm Invasiveness
• Neovascularization, Pathologic (metabolism)
• Nerve Growth Factors (metabolism)
• Nuclear Proteins (metabolism, physiology)
• Serpins (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)
• Urinary Bladder Neoplasms (metabolism, pathology)
• Vascular Endothelial Growth Factor A (metabolism, physiology)
• Young Adult