Upregulation of sFlt-1 by trophoblasts induces the barrier dysfunction of glomerular endothelial cells.

Abstract

This study examined the effect of over-expression of sFlt-1 by trophoblasts on the barrier function of glomerular endothelial cells and the role of VEGF in this process in order to explore the pathogenesis of glomerular disease in preeclampsia. SFlt-1 expression in the human trophoblasts (TEV-1 cells) was enhanced by transfecting sFlt-1 plasmid DNA into TEV-1 cells. The monolayer barrier function of glomerular endothelial cells (ciGEnCs) was determined by measuring the fluorescence intensity of bovine serum albumin (BSA) that crossed the monolayer of glomerular endothelial cells. The results showed that the over-expression of sFlt-1 by TEV-1 cells led to the barrier dysfunction of ciGEnCs, and the exogenous VEGF could alleviate the ciGEnCs dysfunction resulting from the over-expression of sFlt-1 to a certain extent. It was concluded that the dysregulation of sFlt-1 and VEGF in preeclamptic pregnancy may contribute to the barrier dysfunction of glomerular endothelial cells, and VEGF may play an important role in maintaining the barrier function of glomerular endothelial cells, but it may not be the sole factor.

• Authors: Jun Zhao, Haiyi Liu, Hui Du, Fuyuan Qiao, Yvqi Li, Xinwei Shi, Xun Gong, Yuanyuan Wu, Qiong Zhou, Jingjing Xu
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 31 Issue 6 Pg. 815-818 (Dec 2011) ISSN: 1672-0733 [Print] China
• PMID: 22173504 (Publication Type: Journal Article)

Chemical References

• Vascular Endothelial Growth Factor A
• FLT1 protein, human
• Vascular Endothelial Growth Factor Receptor-1

Topics

• Cell Line
• Endothelial Cells (pathology, physiology)
• Female
• Humans
• Kidney Glomerulus (cytology, pathology, physiopathology)
• Pre-Eclampsia (physiopathology)
• Pregnancy
• Trophoblasts (cytology, metabolism)
• Up-Regulation
• Vascular Endothelial Growth Factor A (metabolism)
• Vascular Endothelial Growth Factor Receptor-1 (genetics, metabolism)