Hypocrellin B-mediated sonodynamic action induces apoptosis of hepatocellular carcinoma cells.

Abstract

OBJECTIVE:

The present study aims to investigate apoptosis of hepatocellular carcinoma cells induced by hypocrellin B-mediated sonodynamic action.

METHODS:

The hypocrellin B concentration was kept constant at 2.5 μM and cells from the hepatocellular carcinoma HepG2 cell line were exposed to ultrasound with an intensity of 0.46 W/cm(2) for 8s. Cell cytotoxicity was quantified using an MTT assay 24 h after sonodynamic therapy (SDT) of hypocrellin B. Apoptosis was investigated using a flow cytometry with Annexin V-FITC and propidium iodine staining. Intracellular reactive oxygen species (ROS) levels were detected using a flow cytometry with 2,7-dichlorodihydrofluorecein diacetate (DCFH-DA) staining.

RESULTS:

The cytotoxicity of hypocrellin B-mediated sonodynamic action on HepG2 cells was significantly higher than those of other treatments including ultrasound alone, hypocrellin B alone and sham treatment. Flow cytometry showed that hypocrellin B-induced sonodynamic action markedly enhanced the apoptotic rate of HepG2 cells. Increased ROS was observed in HepG2 cells after being treated with hypocrellin B-mediated sonodynamic action.

CONCLUSIONS:

Our data demonstrated that hypocrellin B-mediated sonodynamic action remarkably induced apoptosis of HepG2 cells, suggesting that apoptosis is an important mechanism of cell death induced by hypocrellin B-mediated SDT.

• Authors: Xinna Wang, Albert Wingnang Leung, Yuan Jiang, Heping Yu, Xiaohong Li, Chuanshan Xu
• Journal: Ultrasonics (Ultrasonics) Vol. 52 Issue 4 Pg. 543-6 (Apr 2012) ISSN: 1874-9968 [Electronic] Netherlands
• PMID: 22172458 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Photosensitizing Agents
• Quinones
• Reactive Oxygen Species
• Perylene
• hypocrellin B

Topics

• Analysis of Variance
• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (therapy)
• Cell Line, Tumor
• Flow Cytometry
• Humans
• Liver Neoplasms (therapy)
• Perylene (analogs & derivatives, pharmacology)
• Photosensitizing Agents (pharmacology)
• Quinones (pharmacology)
• Reactive Oxygen Species (metabolism)
• Staining and Labeling
• Ultrasonic Therapy