A monoclonal antibody against the oncogenic mucin 1 cytoplasmic domain.

Abstract	Mucin 1 (MUC1) is a heterodimeric protein that is aberrantly overexpressed in diverse human carcinomas and certain hematologic malignancies. The transmembrane MUC1-C subunit confers tumorigenicity and is a target for anti-cancer drug development. In this regard, the MUC1-C cytoplasmic domain interacts with multiple effectors that have been linked to transformation. Here we report on the generation of a mouse monoclonal antibody (MAb) against the human MUC1-C cytoplasmic domain (MUC1-CD). This IgG1 MAb, designated anti-MUC1-CD, reacts with the NYGQLDIFP epitope. We show that anti-MUC1-CD is useful in immunoblotting and immunoprecipitation experiments. In addition, anti-MUC1-CD can be used to detect expression of the MUC1-C subunit in formalin-fixed, paraffin-embedded tissues. The MUC1-C inhibitor has entered Phase I evaluation for patients with refractory solid tumors. The present results indicate that the anti-MUC1-CD antibody could be useful as a biomarker to identify patients with tumors that may be responsive to MUC1-C inhibitors.
Authors	Govind Panchamoorthy, Hala Rehan, Akriti Kharbanda, Rehan Ahmad, Donald Kufe
Journal	Hybridoma (2005) (Hybridoma (Larchmt)) Vol. 30 Issue 6 Pg. 531-5 (Dec 2011) ISSN: 1557-8348 [Electronic] United States
PMID	22149278 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References	Antibodies, Monoclonal, Murine-Derived Biomarkers, Tumor Epitopes Immunoglobulin G MUC1 protein, human Mucin-1 Isopropyl Thiogalactoside
Topics	Amino Acid Sequence Animals Antibodies, Monoclonal, Murine-Derived (immunology) Antigen-Antibody Reactions Biomarkers, Tumor (immunology) Cell Line, Tumor Epitope Mapping Epitopes (immunology) Escherichia coli (genetics, metabolism) Humans Hybridomas (immunology) Immunization Immunoblotting Immunoglobulin G (blood, immunology) Immunohistochemistry Immunoprecipitation Isopropyl Thiogalactoside (pharmacology) Mice Mice, Inbred C57BL Molecular Sequence Data Mucin-1 (genetics, immunology) Protein Structure, Tertiary Stomach (immunology) Tissue Fixation (methods)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!