Tristetraprolin down-regulates IL-17 through mRNA destabilization.

Abstract

An excess of interleukin 17 (IL-17) may contribute to chronic inflammatory disorders, but mechanisms that regulate IL-17 in immune cells are unclear. Here we report that tristetraprolin (TTP) inhibits IL-17 production in human T cell lines. Overexpression of TTP decreased the expression of IL-17. Conversely, TTP inhibition by siRNA increased IL-17 production. IL-17 mRNA contains eight AREs within its 3'UTR. TTP bound directly to the IL-17 mRNA 3'UTR at a location between the fourth and seventh AREs and enhanced decay of IL-17 transcripts. These results suggest that TTP could control IL-17-mediated inflammation.

• Authors: Hyun Hee Lee, Nal Ae Yoon, Mai-Tram Vo, Chae Won Kim, Je Moon Woo, Hee Jeong Cha, Young Woo Cho, Byung Ju Lee, Wha Ja Cho, Jeong Woo Park
• Journal: FEBS letters (FEBS Lett) Vol. 586 Issue 1 Pg. 41-6 (Jan 02 2012) ISSN: 1873-3468 [Electronic] England
• PMID: 22138182 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011. Published by Elsevier B.V.

Chemical References

• 3' Untranslated Regions
• Interleukin-17
• RNA, Small Interfering
• Tristetraprolin
• ZFP36 protein, human

Topics

• 3' Untranslated Regions
• Base Sequence
• Binding Sites
• Cell Line
• Down-Regulation
• Gene Expression Regulation
• Humans
• Interleukin-17 (genetics, metabolism)
• Molecular Sequence Data
• RNA Stability
• RNA, Small Interfering
• T-Lymphocytes (metabolism)
• Tristetraprolin (genetics, metabolism)