Abstract |
Adoptive T cell therapy for cancer patients optimally requires participation of CD4 T cells. In this phase I/II study, we assessed the therapeutic effects of adoptively transferred IL-10- and IFN-γ-producing CD4 effector cells in patients with recurrent ovarian cancer. Using MUC1 peptide and IL-2 for ex vivo CD4 effector cell generation, we show that three monthly treatment cycles of autologous T cell restimulation and local intraperitoneal re-infusion-modulated T cell-mediated immune responses that were associated with enhanced patient survival. One patient remains disease-free, another patient experienced prolonged survival for nearly 16 months with recurrent disease, and two patients expired within 3-5 months following final infusion. Prolonged survivors showed elevated levels of systemic CD3(+)CD4(+)CD25(+) and CD3(+)CD4(+)CD25(-) T cells when compared to that of pre-treatment levels and similarly treated short-term survivors. Such cell populations among these patients contained variable levels of "Inducible" Tr1 (CD4(+)CD25(-)FoxP3(-)IL-10(+)) and "Natural" (CD4(+)CD25(+)CD45RO(+)FoxP3(+)) TReg cell numbers and ratios that were associated with prolonged and/or disease-free survival. Moreover, peptide-restimulated T cells from these patients showed an elevation in both IFN-γ production, memory cell phenotype, and select TNF family ligands associated with enhanced T cell survival and apoptosis-inducing activities. This suggests that intraperitoneally administered Th1-like cells, producing elevated levels of IL-10, may require and/or induce differential levels of distinct systemic TReg subpopulations that influence, in part, long-term tumor immunity and enhanced memory/effector CD4-mediated therapeutic potentials. Furthermore, treatment efficacy and enhanced memory cell phenotype did not appear to be dependent on TReg cell numbers but upon ratios of "Inducible" and "Natural" TReg subpopulations.
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Authors | Mark J Dobrzanski, Kathleen A Rewers-Felkins, Khaliquzzaman A Samad, Imelda S Quinlin, Catherine A Phillips, William Robinson, David J Dobrzanski, Stephen E Wright |
Journal | Cancer immunology, immunotherapy : CII
(Cancer Immunol Immunother)
Vol. 61
Issue 6
Pg. 839-54
(Jun 2012)
ISSN: 1432-0851 [Electronic] Germany |
PMID | 22083345
(Publication Type: Clinical Trial, Phase I, Clinical Trial, Phase II, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Mucin-1
- Interleukin-10
- Interferon-gamma
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Topics |
- Aged
- Female
- Humans
- Immunotherapy
- Interferon-gamma
(biosynthesis, immunology)
- Interleukin-10
(immunology)
- Middle Aged
- Mucin-1
(immunology)
- Ovarian Neoplasms
(genetics, immunology, therapy)
- Phenotype
- T-Lymphocytes, Regulatory
(immunology)
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