Tissue microarrays in Chinese human rectal cancer: study of expressions of the tumor-associated genes.

Abstract

BACKGROUNDS/AIMS:

The cellular basis for rectal cancer development is still unclear. The aim of this study was to evaluate the relationship between the expression of p53, cyclinD1, bcl-2, ß-catenin, c-myc, cyclooxygenase-2 (COX-2) and nm23-H1 and the clinicopathological characteristics of rectal cancer.

METHODOLOGY:

Expressions of p53, cyclinD1, bcl-2, ß-catenin, c-myc, COX-2 and nm23-H1 proteins were detected by immunohistochemical staining to two tissue microarrays containing tissues accumulated from 54 human rectal cancers and 40 para-cancer mucosa.

RESULTS:

Significant differences were demonstrated between the rectal cancers and their benign para-cancer counterparts according to the expressions of p53, cyclinD1, bcl-2, ß-catenin, c-myc, COX-2 and nm23-H1 (p<0.05). Additionally, positive correlations of ß-catenin with cyclinD1 and c-myc (r=0.412, p=0.002; r=0.447, p=0.000) and of p53 with bcl-2 (r=0.332, p=0.001) were found. Cancer tissues with overexpression of ß-catenin or bcl-2 were less likely to differentiate to advanced grade. Expression of cyclinD1 had a correlation with clinical stages (p=0.039). In addition, a negative correlation was found between nm23-H1 expression and the histological grades, distance metastasis and Duke's stages.

CONCLUSIONS:

Aberrant expression of p53, cyclinD1, bcl-2, ß-catenin, c-myc, COX-2 and nm23-H1 might attribute to the carcinogenesis of human rectal cancer. Furthermore, cyclinD1 and nm23-H1 might be involved in rectal cancer progression. This study recommends the application of tissue microarrays in rectal cancer research for its reliable quick throughput.

• Authors: Mao Song Lin, Wei Chang Chen, Jun Xing Huang, Heng Jun Gao, Bao Feng Zhang, Jing Fang, Qiong Zhou, Ying Hu
• Journal: Hepato-gastroenterology (Hepatogastroenterology) 2011 Nov-Dec Vol. 58 Issue 112 Pg. 1937-42 ISSN: 0172-6390 [Print] Greece
• PMID: 22024062 (Publication Type: Journal Article)

Chemical References

• CCND1 protein, human
• CTNNB1 protein, human
• MYC protein, human
• Proto-Oncogene Proteins c-bcl-2
• Proto-Oncogene Proteins c-myc
• beta Catenin
• Cyclin D1

Topics

• Adult
• Aged
• Aged, 80 and over
• Cyclin D1 (analysis, genetics)
• Female
• Genes, p53
• Humans
• Immunohistochemistry
• Intestinal Mucosa (metabolism)
• Male
• Middle Aged
• Proto-Oncogene Proteins c-bcl-2 (analysis, genetics)
• Proto-Oncogene Proteins c-myc (analysis, genetics)
• Rectal Neoplasms (chemistry, metabolism, pathology)
• Tissue Array Analysis (methods)
• beta Catenin (analysis, genetics)