Identification of four isoforms of aldolase B down-regulated in hepatocellular carcinoma tissues by means of two-dimensional Western blotting.

Abstract

BACKGROUND:

Hepatocellular carcinoma (HCC) is one of the most lethal diseases, and one of the major causes of death in Japan. Our previous research of proteomics for cancerous and paired non-cancerous tissues from patients with HCC with hepatitis C virus infection (HCV-HCC) by means of the combination of two-dimensional gel electrophoresis (2-DE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) reported that four of numerous spots of weaker intensity in cancerous tissues than in paired non-cancerous tissues were identified as four isoforms of liver type aldolase (aldolase B). In the present study, two-dimensional (2-D) Western blot analysis demonstrated a significantly lower expression of four isoforms of aldolase B in cancerous than in non-cancerous tissues.

CONCLUSION:

Our finding of differences of expression aldolase B isoforms between cancerous and paired non-cancerous tissues for HCV-HCC may be useful for shedding light on some behaviors of aldolase B during hepatocellular carcinogenesis.

• Authors: Yufeng Wang, Yasuhiro Kuramitsu, Motonari Takashima, Yuichiro Yokoyama, Norio Iizuka, Takao Tamesa, Isao Sakaida, Masaaki Oka, Kazuyuki Nakamura
• Journal: In vivo (Athens, Greece) (In Vivo) 2011 Nov-Dec Vol. 25 Issue 6 Pg. 881-6 ISSN: 1791-7549 [Electronic] Greece
• PMID: 22021680 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Isoenzymes
• Fructose-Bisphosphate Aldolase

Topics

• Blotting, Western (methods)
• Carcinoma, Hepatocellular (enzymology, pathology)
• Chromatography, Liquid
• Down-Regulation
• Electrophoresis, Gel, Two-Dimensional
• Fructose-Bisphosphate Aldolase (metabolism)
• Humans
• Isoenzymes (metabolism)
• Liver Neoplasms (enzymology, pathology)
• Tandem Mass Spectrometry