[Effect of polypeptide extract from scorpion venom (PESV) with chemotherapy inhibited angiogenesis of Lewis lung carcinomas].

Abstract

OBJECTIVE:

To study the effects of polypeptide extract from scorpion venom (PESV) alliance with chemotherapy on angiogenesis of Lewis lung carcinomas (LLC) and its mechanism.

METHOD:

LLC cells suspension (4 x 10(6) cells/mL) were subcutaneously injected into 54 C57BL/6J mice in right armpits. Then the tumor-bearing mice were randomly divided into three groups: the control group, the chemotherapy group and the PESV group. Cyclophosphamide was used to establish the model of cancer. Chemotherapy and PESV were added to the PESV group. Every 7 days, 6 mice of each group were executed, and the experiments were carried out for 28 days. The tumor volume and inhibitory rate were determined. Immunohistochemistry and RT-PCR were used to determine the expression of factor VIII, alpha-SMA, Dll4 and Notch1 in tumor tissue. Correlation analysis was used to identify the relationship of factor VIII and calculate microvessel density (MVD), alpha-SMA and vascular maturity.

RESULT:

The inhibitory rate of PESV was 42.21%. Comparing with the chemotherapy group, the expression of tumor factor Dll4 and Notch1 in the PESV group were decreased significantly (P < 0.05). The expression of factor VIII and alpha-SMA in the chemotherapy group is lower than the control group (P < 0.05), while it's higher when compared with the PESV group (P < 0.01). Expression of Dll4 and Notch1 in the chemotherapy group at the 28th day were higher than the control group (P < 0.05), and the expression in the PESV group at the 21st day were significantly lower than the chemotherapy group (P < 0.05).

CONCLUSION:

PESV could inhibit the angiogenesis of LLC. It might be attained by decreasing the level of angiogenic factors, that are factor VIII, alpha-SMA, Dll4 and Notch1 in tumor microenvironment.

• Authors: Xiaojia Sun, Yueying Zhang, Qing Jia, Zhaopeng Wang, Zhaoxia Wang, Weidong Zhang
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 36 Issue 12 Pg. 1644-9 (Jun 2011) ISSN: 1001-5302 [Print] China
• PMID: 22007553 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents
• Peptides
• Scorpion Venoms

Topics

• Animals
• Antineoplastic Agents (blood, chemistry, therapeutic use)
• Carcinoma, Lewis Lung (drug therapy)
• Immunohistochemistry
• Male
• Mice
• Mice, Inbred C57BL
• Neovascularization, Pathologic (drug therapy)
• Peptides (chemistry, therapeutic use)
• Reverse Transcriptase Polymerase Chain Reaction
• Scorpion Venoms (chemistry)