The limited expression of N-Glycolyl GM3 (NeuGcGM3)
ganglioside in human normal tissues, as well as its presence in
melanoma and
breast carcinoma using 14F7 Mab (anti-NeuGcGM3), has been previously reported. In this work we evaluated for the first time the 14F7 Mab immunorecognition in some digestive system
tumors. Immunohistochemical assays were made with 14F7, followed by anti-mouse biotinylated antibody and ABC/HRP system in normal and pathological human tissues were made. No immunoreaction was evidenced in normal tissues. The reactivity of 14F7 was detected in all
adenocarcinomas of the stomach (12/12), colon (12/12), and pancreas (11/11). A finely granular immunorecognition in esophageal
tumors (5/15),
epidermoid carcinoma of the rectum (5/7), and basaloid
carcinoma (4/5) of the latter as well as in
hepatocellular carcinoma (13/14) was also observed. Our results are in agreement with the assumption that NeuGcGM3
ganglioside may be considered as target for passive and active immunotherapy in digestive system
malignancies expressing this molecule.