[Nerve growth factor increases sodium current via interferon regulatory factor-1 pathway in rat pheochromocytoma cells].

Abstract

OBJECTIVE:

To explore the effect of nerve growth factor(NGF) and interferon regulatory factor-1(IRF-1) on sodium current change of sensory neuron in rat pheochromocytoma cells.

METHODS:

Sensory neuron rat pheochromocytoma cells were stimulated by different concentrations of NGF(0-200 ng/mL), the IRF-1 mRNA levels were examined by real-time PCR, and the activation of IRF-1 was examined by Western blot. The sodium current change was recorded by patch clamp.

RESULTS:

Low concentration of NGF improved the sodium current, which was concentration dependent. When exposed to high concentration of NGF, the expression of IRF-1 mRNA in PC-12 was improved. Low concentration of NGF resulted in IRF-1 intronuclear transporting, and the expression was not affected. Sodium current did not occur in PC-12 cells when IRF-1 was blocked.

CONCLUSION:

NGF can improve the sodium current in PC-12 cells concentration-dependently, and the improvement is regulated by IRF-1.

• Authors: Yemu Zhu, Pinhua Pan, Hongyi Tan, Chengping Hu
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 36 Issue 8 Pg. 776-81 (Aug 2011) ISSN: 1672-7347 [Print] China
• PMID: 21937806 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interferon Regulatory Factor-1
• RNA, Messenger
• Sodium Channels
• Nerve Growth Factor

Topics

• Animals
• Interferon Regulatory Factor-1 (genetics, metabolism)
• Nerve Growth Factor (pharmacology)
• PC12 Cells
• RNA, Messenger (genetics, metabolism)
• Rats
• Real-Time Polymerase Chain Reaction
• Sodium Channels (drug effects)