Expanded CUG repeats Dysregulate RNA splicing by altering the stoichiometry of the muscleblind 1 complex.

Abstract

To understand the role of the splice regulator muscleblind 1 (MBNL1) in the development of RNA splice defects in myotonic dystrophy I (DM1), we purified RNA-independent MBNL1 complexes from normal human myoblasts and examined the behavior of these complexes in DM1 myoblasts. Antibodies recognizing MBNL1 variants (MBNL1(CUG)), which can sequester in the toxic CUG RNA foci that develop in DM1 nuclei, were used to purify MBNL1(CUG) complexes from normal myoblasts. In normal myoblasts, MBNL1(CUG) bind 10 proteins involved in remodeling ribonucleoprotein complexes including hnRNP H, H2, H3, F, A2/B1, K, L, DDX5, DDX17, and DHX9. Of these proteins, only MBNL1(CUG) colocalizes extensively with DM1 CUG foci (>80% of foci) with its partners being present in <10% of foci. Importantly, the stoichiometry of MBNL1(CUG) complexes is altered in DM1 myoblasts, demonstrating an increase in the steady state levels of nine of its partner proteins. These changes are recapitulated by the expression of expanded CUG repeat RNA in Cos7 cells. Altered stoichiometry of MBNL1(CUG) complexes results from aberrant protein synthesis or stability and is unlinked to PKCα function. Modeling these changes in normal myoblasts demonstrates that increased levels of hnRNP H, H2, H3, F, and DDX5 independently dysregulate splicing in overlapping RNA subsets. Thus expression of expanded CUG repeats alters the stoichiometry of MBNL1(CUG) complexes to allow both the reinforcement and expansion of RNA processing defects.

• Authors: Sharan Paul, Warunee Dansithong, Sonali P Jog, Ian Holt, Saloni Mittal, J David Brook, Glenn E Morris, Lucio Comai, Sita Reddy
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 286 Issue 44 Pg. 38427-38438 (Nov 04 2011) ISSN: 1083-351X [Electronic] United States
• PMID: 21900255 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Heterogeneous-Nuclear Ribonucleoproteins
• MBNL1 protein, human
• RNA, Small Interfering
• RNA-Binding Proteins

Topics

• Animals
• COS Cells
• Cell Line
• Chlorocebus aethiops
• Heterogeneous-Nuclear Ribonucleoproteins (metabolism)
• Humans
• Immunoprecipitation
• Mass Spectrometry (methods)
• Muscle, Skeletal (metabolism)
• Myotonic Dystrophy (genetics, metabolism)
• Protein Interaction Mapping (methods)
• RNA Splicing
• RNA, Small Interfering (metabolism)
• RNA-Binding Proteins (metabolism)
• Subcellular Fractions