Cathepsin B, D, and L regulation in cyclosporin A-mediated gingival hyperplasia of a patient with sarcoidosis.

Abstract

BACKGROUND:

Cyclosporin A (CsA) is an immunosuppressant with side effects including gingival hyperplasia. Sarcoidosis is a systemic disease characterized by granulomas. Here, we report on a rare case of sarcoidosis with gingival hyperplasia to clarify whether clinical observation corresponds to in vitro results.

METHODS:

Gingival fibroblasts (HGFs) were isolated from healthy gingiva and cultured with CsA. Total RNA was collected and expression of mRNAs examined using semi-quantitative RT-PCR analysis. Cathepsin B, D, and L expression in overgrown gingiva of the patient was examined by immunohistochemistry.

RESULTS:

Cathepsin D, L, and vascular endothelial growth factor (VEGF)165 mRNA were markedly suppressed in CsA-treated HGFs, whereas cathepsin B, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA were not reduced. Next, the decrease of cathepsin B and L expression in enlarged gingiva was observed, whereas an increase of cathepsin D expression was observed. Clinically, the enlarged gingival lesions were fully resolved by performing oral infection control.

CONCLUSIONS:

Cathepsins regulation might be an important factor in the development of CsA-mediated gingival hyperplasia.

• Authors: Osamu Murai, Koji Naruishi, Satoshi Ogihara, Nagisa Suwa, Satomi Kanazawa, Takashi Yaegashi, Yasunori Takeda, Kazushi Kunimatsu
• Journal: Clinical laboratory (Clin Lab) Vol. 57 Issue 7-8 Pg. 535-41 ( 2011) ISSN: 1433-6510 [Print] Germany
• PMID: 21888018 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adrenal Cortex Hormones
• Immunosuppressive Agents
• TIMP1 protein, human
• Tissue Inhibitor of Metalloproteinase-1
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• Cyclosporine
• CTSB protein, human
• Cathepsin B
• CTSL protein, human
• Cathepsin L
• CTSD protein, human
• Cathepsin D
• Matrix Metalloproteinase 1

Topics

• Adrenal Cortex Hormones (administration & dosage, therapeutic use)
• Bacteroidaceae Infections (complications)
• Cathepsin B (biosynthesis, genetics)
• Cathepsin D (biosynthesis, genetics)
• Cathepsin L (biosynthesis, genetics)
• Cyclosporine (administration & dosage, adverse effects, pharmacology, therapeutic use)
• Dental Scaling
• Drug Therapy, Combination
• Enzyme Induction (drug effects)
• Female
• Gene Expression Regulation (drug effects)
• Gingival Hyperplasia (chemically induced, etiology, metabolism, prevention & control)
• Gingivitis (complications, microbiology, therapy)
• Humans
• Immunocompromised Host
• Immunosuppressive Agents (administration & dosage, adverse effects, pharmacology, therapeutic use)
• Matrix Metalloproteinase 1 (biosynthesis, genetics)
• Middle Aged
• Oral Hygiene
• Porphyromonas gingivalis (isolation & purification)
• Sarcoidosis (complications, drug therapy)
• Tissue Inhibitor of Metalloproteinase-1 (biosynthesis, genetics)
• Treponema denticola (isolation & purification)
• Vascular Endothelial Growth Factor A (biosynthesis, genetics)