Enhanced sucrose and cocaine self-administration and cue-induced drug seeking after loss of VGLUT2 in midbrain dopamine neurons in mice.

Abstract

The mesostriatal dopamine (DA) system contributes to several aspects of responses to rewarding substances and is implicated in conditions such as drug addiction and eating disorders. A subset of DA neurons has been shown to express the type 2 Vesicular glutamate transporter (Vglut2) and may therefore corelease glutamate. In the present study, we analyzed mice with a conditional deletion of Vglut2 in DA neurons (Vglut2(f/f;DAT-Cre)) to address the functional significance of the glutamate-DA cophenotype for responses to cocaine and food reinforcement. Biochemical parameters of striatal DA function were also examined by using DA receptor autoradiography, immediate-early gene quantitative in situ hybridization after cocaine challenge, and DA-selective in vivo chronoamperometry. Mice in which Vglut2 expression had been abrogated in DA neurons displayed enhanced operant self-administration of both high-sucrose food and intravenous cocaine. Furthermore, cocaine seeking maintained by drug-paired cues was increased by 76%, showing that reward-dependent plasticity is perturbed in these mice. In addition, several lines of evidence suggest that adaptive changes occurred in both the ventral and dorsal striatum in the absence of VGLUT2: DA receptor binding was increased, and basal mRNA levels of the DA-induced early genes Nur77 and c-fos were elevated as after cocaine induction. Furthermore, in vivo challenge of the DA system by potassium-evoked depolarization revealed less DA release in both striatal areas. This study demonstrates that absence of VGLUT2 in DA neurons leads to perturbations of reward consumption as well as reward-associated memory, features of particular relevance for addictive-like behavior.

• Authors: Johan Alsiö, Karin Nordenankar, Emma Arvidsson, Carolina Birgner, Souha Mahmoudi, Briac Halbout, Casey Smith, Guillaume M Fortin, Lars Olson, Laurent Descarries, Louis-Éric Trudeau, Klas Kullander, Daniel Lévesque, Asa Wallén-Mackenzie
• Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (J Neurosci) Vol. 31 Issue 35 Pg. 12593-603 (Aug 31 2011) ISSN: 1529-2401 [Electronic] United States
• PMID: 21880920 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dopamine Plasma Membrane Transport Proteins
• Dopamine Uptake Inhibitors
• Nr4a1 protein, mouse
• Nuclear Receptor Subfamily 4, Group A, Member 1
• Proto-Oncogene Proteins c-fos
• RNA, Messenger
• Receptors, Dopamine
• Slc17a6 protein, mouse
• Vesicular Glutamate Transport Protein 2
• Sucrose
• Potassium Chloride
• Cocaine
• Dopamine

Topics

• Analysis of Variance
• Animals
• Autoradiography
• Behavior, Addictive (genetics, physiopathology)
• Behavior, Animal
• Cell Death (drug effects, genetics, immunology)
• Cocaine (administration & dosage)
• Conditioning, Operant (drug effects, physiology)
• Cues
• Dopamine (metabolism)
• Dopamine Plasma Membrane Transport Proteins (genetics)
• Dopamine Uptake Inhibitors (administration & dosage)
• Electrochemical Techniques (methods)
• Food Preferences (drug effects, physiology)
• Gene Expression Regulation (drug effects, genetics)
• Male
• Mesencephalon (cytology, drug effects, metabolism)
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Neurons (metabolism)
• Nuclear Receptor Subfamily 4, Group A, Member 1 (genetics, metabolism)
• Potassium Chloride (pharmacology)
• Protein Binding (genetics)
• Proto-Oncogene Proteins c-fos (genetics, metabolism)
• RNA, Messenger (metabolism)
• Receptors, Dopamine (metabolism)
• Reinforcement Schedule
• Reward
• Self Administration (methods)
• Sucrose (administration & dosage)
• Vesicular Glutamate Transport Protein 2 (deficiency)