Abstract | BACKGROUND:
Serum protein profiles have been investigated frequently to discover early biomarkers for breast cancer. So far, these studies used biological samples collected at or after diagnosis. This may limit these studies' value in the search for cancer biomarkers because of the often advanced tumor stage, and consequently risk of reverse causality. We present for the first time pre-diagnostic serum protein profiles in relation to breast cancer, using the Prospect-EPIC (European Prospective Investigation into Cancer and nutrition) cohort. METHODS: In a nested case-control design we compared 68 women diagnosed with breast cancer within three years after enrollment, with 68 matched controls for differences in serum protein profiles. All samples were analyzed with SELDI-TOF MS (surface enhanced laser desorption/ionization time-of-flight mass spectrometry). In a subset of 20 case-control pairs, the serum proteome was identified and relatively quantified using isobaric Tags for Relative and Absolute Quantification (iTRAQ) and online two-dimensional nano-liquid chromatography coupled with tandem MS (2D-nanoLC-MS/MS). RESULTS: CONCLUSIONS:
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Authors | Annemieke W J Opstal-van Winden, Esmeralda J M Krop, Monica H Kåredal, Marie-Christine W Gast, Christian H Lindh, Marina C Jeppsson, Bo A G Jönsson, Diederick E Grobbee, Petra H M Peeters, Jos H Beijnen, Carla H van Gils, Roel C H Vermeulen |
Journal | BMC cancer
(BMC Cancer)
Vol. 11
Pg. 381
(Aug 26 2011)
ISSN: 1471-2407 [Electronic] England |
PMID | 21871081
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Aged
- Biomarkers, Tumor
(blood)
- Breast Neoplasms
(diagnosis)
- Case-Control Studies
- Female
- Humans
- Middle Aged
- Proteomics
- Risk Factors
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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