Abstract | AIM: MATERIALS AND METHODS: The effect of DTIC and imexon, alone and in combination, was evaluated for growth inhibition (MTT), radiolabeled drug uptake, cellular thiol content (HPLC), and DNA strand breaks (Comet assay). Pharmacokinetic and antitumor effects were evaluated in mice. RESULTS: Growth inhibition in vitro was additive with the two drugs. There was no effect on drug uptake or on the number of DNA strand breaks. There was a >75% reduction in cellular glutathione and cysteine with imexon but not DTIC. Co-administration of the two drugs in mice caused an increase in the area under the curve of both drugs, but the combination was not effective in reducing human A375 melanoma tumors in vivo. CONCLUSION:
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Authors | Betty K Samulitis, Robert T Dorr, H-H Sherry Chow |
Journal | Anticancer research
(Anticancer Res)
Vol. 31
Issue 9
Pg. 2781-5
(Sep 2011)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 21868520
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Antineoplastic Agents
- Hexanones
- 4-imino-1,3-diazabicyclo(3.1.0)hexan-2-one
- Dacarbazine
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Topics |
- Animals
- Antineoplastic Agents
(blood, pharmacokinetics, pharmacology)
- Area Under Curve
- Cell Line, Tumor
- Comet Assay
- Dacarbazine
(blood, pharmacokinetics, pharmacology)
- Drug Interactions
- Drug Screening Assays, Antitumor
- Hexanones
(blood, pharmacokinetics, pharmacology)
- Humans
- In Vitro Techniques
- Male
- Melanoma
(pathology)
- Mice
- Mice, SCID
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