Systemic heme oxygenase-1 transgenic overexpression aggravates pressure overload-induced cardiac hypertrophy in mice.

Abstract

BACKGROUND/AIMS:

Heme oxygenase-1(HO-1) has been reported to protect against cardiac hypertrophy in cultured neonatal cardiomyocytes treated with HO-1 inducer, cardiac specific HO-1 transgenic mice, or animals treated with HO-1 inducer. The aim of the present study is to examine the effects of systemic HO-1 transgenic overexpression on pressure overload-induced cardiac hypertrophy in mice.

METHODS:

Pressure-overload cardiac hypertrophy was induced by transverse aortic constriction (TAC) in WT (wild type) and systemic HO-1 transgenic overexpression (TG) mice.

RESULTS:

We found that systemic HO-1 transgenic overexpression aggravated pressure overload-induced cardiac hypertrophy. Pressure-overload induced the more increases of heart weight/ body weigh index, left ventricular weight/ body weight index, β-MHC protein expression, cardiac interstitial fibrosis in TG mice than in WT mice. Pressure-overload increased cardiac HO-1 protein expression in WT but not TG mice, but the cardiac HO-1 protein level was still higher in TAC-treated TG mice than in TAC-treated WT mice. The basal cardiac calcineurin protein level in TG mice was lower than that in WT mice. Pressure-overload increased calcineurin protein expression in both WT and TG mice; however, pressure-overload induced more calcineurin protein expression in TG mice than in WT mice.

CONCLUSION:

This study shows for the first time that systemic HO-1 transgenic overexpression aggravates pressure overload-induced cardiac hypertrophy.

• Authors: Chang Chen, Rong Huo, Yan Tong, Yue Sheng, Hui-Bin Liu, Xu Gao, Osamu Nakajima, Bao-Feng Yang, De-Li Dong
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 28 Issue 1 Pg. 25-32 ( 2011) ISSN: 1421-9778 [Electronic] Germany
• PMID: 21865845 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 S. Karger AG, Basel.

Chemical References

• Myh7 protein, mouse
• Iron
• Heme Oxygenase (Decyclizing)
• Heme Oxygenase-1
• heme oxygenase-2
• Calcineurin
• Myosin Heavy Chains

Topics

• Animals
• Aorta (physiopathology)
• Calcineurin (metabolism)
• Cardiomegaly (enzymology, etiology, metabolism, pathology)
• Constriction, Pathologic (physiopathology)
• Disease Models, Animal
• Fibrosis (etiology)
• Gene Expression
• Heme Oxygenase (Decyclizing) (metabolism)
• Heme Oxygenase-1 (genetics, metabolism)
• Hemodynamics (physiology)
• Iron (analysis)
• Mice
• Mice, Transgenic
• Myosin Heavy Chains (metabolism)