Inhibition of LPS-induced inflammatory biomarkers by ethyl acetate fraction of Patrinia scabiosaefolia through suppression of NF-κB activation in RAW 264.7 cells.

Abstract

Patrinia scabiosaefolia (PS) has been used for curing various types of inflammatory-related disorders. However, the precise mechanism of the anti-inflammatory activity of PS remains unclear. Here, we investigated the anti-inflammatory effects of several fractions isolated from the PS in RAW 264.7 macrophages. The results indicated that the ethyl acetate fraction of PS (EAPS) concentration highly suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) and IL-6 productions without a cytotoxic effect on RAW 264.7 cells. EAPS inhibited the expressions of LPS-induced iNOS and COX-2 protein and their mRNA in a dose-dependent manner. Particularly, EAPS suppressed the level of nuclear factor-κB (NF-κB) activity, which was linked with the suppression of LPS-induced phosphorylation of p65 at serine 276 and p65 translocation into nuclei, but not MAPK signaling. In addition, treatment with EAPS inhibited the production of TNF-α in LPS-injected mice and suppressed the production of IL-6 and TNF-α in LPS-stimulated splenocytes from BALB/c mice. Therefore, we demonstrate here that Patrinia scabiosaefolia potentially inhibits the biomarkers related to inflammation through the blocking of NF-κB p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases.

• Authors: Eun-Jung Lee, Chulwon Kim, Jin-Young Kim, Sung-Moo Kim, Dongwoo Nam, Hyeung-Jin Jang, Sung-Hoon Kim, Bum Sang Shim, Kyoo Seok Ahn, Seung-Hoon Choi, Sang Hoon Jung, Kwang Seok Ahn
• Journal: Immunopharmacology and immunotoxicology (Immunopharmacol Immunotoxicol) Vol. 34 Issue 2 Pg. 282-91 (Apr 2012) ISSN: 1532-2513 [Electronic] England
• PMID: 21854107 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetates
• Biomarkers
• Interleukin-6
• Lipopolysaccharides
• NF-kappa B
• Plant Extracts
• Rela protein, mouse
• Transcription Factor RelA
• Tumor Necrosis Factor-alpha
• Nitric Oxide
• ethyl acetate
• Nitric Oxide Synthase Type II
• Nos2 protein, mouse
• Ptgs2 protein, mouse
• Cyclooxygenase 2
• Extracellular Signal-Regulated MAP Kinases
• JNK Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases

Topics

• Acetates (chemistry)
• Animals
• Biomarkers (blood, metabolism)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Cyclooxygenase 2 (genetics, metabolism)
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Gene Expression (drug effects, genetics)
• Inflammation (metabolism)
• Interleukin-6 (metabolism)
• JNK Mitogen-Activated Protein Kinases (metabolism)
• Lipopolysaccharides (pharmacology)
• Lymphocytes (drug effects, metabolism)
• MAP Kinase Signaling System (drug effects)
• Macrophages (drug effects, metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• NF-kappa B (metabolism)
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (genetics, metabolism)
• Patrinia (chemistry)
• Phosphorylation (drug effects)
• Plant Extracts (administration & dosage, isolation & purification, pharmacology)
• Spleen (cytology)
• Transcription Factor RelA (metabolism)
• Tumor Necrosis Factor-alpha (blood, metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)