Development of autoimmune diabetes in the absence of detectable IL-17A in a CD8-driven virally induced model.

Abstract

Recent studies have shown that IL-17 can contribute beneficially to pathogen defense but also that excessive IL-17 levels are associated with chronic inflammation and autoimmune disorders. To date, the role of IL-17 in viral infections and type 1 diabetes is ambiguous. In this study, we used IL-17A enhanced green fluorescent protein bicistronic reporter mouse strains to analyze in situ production of IL-17A. Upon Klebsiella pneumoniae bacterial infection, CD4(+) and γδ T cells produce IL-17A. In contrast, CD4(+) or CD8(+) T cells do not produce IL-17A in response to acute or protracted viral infection with lymphocytic choriomeningitis virus or during autoimmune diabetes development in the CD8-driven lymphocytic choriomeningitis virus-induced model of type 1 diabetes. We conclude that viral elimination and type 1 diabetes can occur in the absence of detectable IL-17A production, suggesting IL-17A is not essential in these settings.

• Authors: Tom L Van Belle, Enric Esplugues, Jeanette Liao, Therese Juntti, Richard A Flavell, Matthias G von Herrath
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 187 Issue 6 Pg. 2915-22 (Sep 15 2011) ISSN: 1550-6606 [Electronic] United States
• PMID: 21832162 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-17
• Green Fluorescent Proteins

Topics

• Animals
• CD4-Positive T-Lymphocytes
• CD8-Positive T-Lymphocytes (immunology)
• Cell Separation
• Diabetes Mellitus, Type 1 (immunology)
• Disease Models, Animal
• Female
• Flow Cytometry
• Gene Knock-In Techniques
• Genes, Reporter
• Green Fluorescent Proteins (genetics)
• Interleukin-17 (immunology)
• Lymphocytic choriomeningitis virus (immunology)
• Male
• Mice
• Mice, Inbred C57BL
• Virus Diseases (immunology)