Immunohistochemical expression of nuclear factor κB, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts.

Abstract

OBJECTIVE:

The aim of this study was to compare the immunohistochemical expression of nuclear factor κB (NF-κB), matrix metalloproteinase 9 (MMP-9), and CD105 in odontogenic keratocysts (OKCs), dentigerous cysts (DCs), and radicular cysts (RCs).

STUDY DESIGN:

Twenty cases of OKCs, 20 DCs, and 20 RCs were analyzed. A labeling index (LI), which expresses the percentage of NF-κB-stained nuclei, was calculated for the analysis of NF-κB expression. Expression of MMP-9 in the epithelium and in the capsule of each lesion was scored as 0 (<10% stained cells), 1 (10%-50% stained cells), or 2 (>50% stained cells). In addition, MMP-9 immunostaining was analyzed in endothelial cells of vessels with a conspicuous lumen. The angiogenic index was determined based on the number of anti-CD105 antibody-stained microvessels.

RESULTS:

In the epithelial component, the NF-κB LI was higher in OKCs than in DCs and RCs (P < .001). Analysis of MMP-9 expression in the epithelial component showed a predominance of score 2 in OKCs (90%), DCs (70%), and RCs (65%; P = .159). Evaluation of the NF-κB LI according to the expression of MMP-9 in the epithelial lining revealed no significant difference between lesions (P = .282). In the fibrous capsule, the highest percentage of MMP-9-stained cells (score 2) was observed in OKCs (P = .100). Analysis of the expression of MMP-9 in the vessels of odontogenic cysts showed a predominance of score 2 in OKCs (80%) and RCs (50%) and of score 1 in DCs (75%; P = .002). Mean microvessel count was high in RCs (16.9), followed by DCs (12.1) and OKCs (10.0; P = .163). No significant difference in microvessel count according to the expression of MMP-9 was observed between groups (P = .689).

CONCLUSIONS:

The results suggest that the more aggressive biologic behavior of OKCs is related to the higher expression of MMP-9 and NF-κB in those lesions. The differences in the biologic behavior of the lesions studied do not seem to be associated with the angiogenic index.

• Authors: Pedro Paulo de Andrade Santos, Ana Rafaela Luz de Aquino, Alessandra Oliveira Barreto, Roseana de Almeida Freitas, Hébel Cavalcanti Galvão, Lélia Batista de Souza
• Journal: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (Oral Surg Oral Med Oral Pathol Oral Radiol Endod) Vol. 112 Issue 4 Pg. 476-83 (Oct 2011) ISSN: 1528-395X [Electronic] United States
• PMID: 21821445 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Mosby, Inc. All rights reserved.

Chemical References

• Antigens, CD
• Coloring Agents
• ENG protein, human
• Endoglin
• NF-kappa B
• Receptors, Cell Surface
• Matrix Metalloproteinase 9

Topics

• Antigens, CD (analysis)
• Cell Nucleus (pathology)
• Coloring Agents
• Dentigerous Cyst (pathology)
• Endoglin
• Endothelial Cells (pathology)
• Endothelium, Vascular (pathology)
• Epithelium (pathology)
• Humans
• Immunohistochemistry
• Matrix Metalloproteinase 9 (analysis)
• Microvessels (pathology)
• NF-kappa B (analysis)
• Neovascularization, Pathologic (pathology)
• Odontogenic Cysts (pathology)
• Radicular Cyst (pathology)
• Receptors, Cell Surface (analysis)