Effects of oleanolic acid on pulmonary morphofunctional and biochemical variables in experimental acute lung injury.

Abstract

We analysed the effects of oleanolic acid (OA) on lung mechanics and histology and its possible mechanisms of action in experimental acute lung injury (ALI). BALB/c mice were randomly divided into Control (saline, ip) and ALI (paraquat, 25 mg/kg, ip) groups. At 1 h, both groups were treated with saline (SAL, 50 μl ip), OA (10 mg/kg ip), or dexamethasone (DEXA, 1 mg/kg ip). At 24 h, lung static elastance, viscoelastic pressure, and alveolar collapse reduced more after OA compared to DEXA administration. Tumour necrosis factor-α, macrophage migration inhibitory factor, interleukin-6, interferon-γ, and transforming growth factor-β mRNA expressions in lung tissue diminished similarly after OA or DEXA. Conversely, only OA avoided reactive oxygen species generation and yielded a significant decrease in nitrite concentration. OA and DEXA restored the reduced glutathione/oxidized glutathione ratio and catalase activity while increasing glutathione peroxidase induced by paraquat. In conclusion, OA improved lung morphofunction by modulating the release of inflammatory mediators and oxidative stress.

• Authors: Raquel S Santos, Pedro L Silva, Gisele P Oliveira, Fernanda F Cruz, Débora S Ornellas, Marcelo M Morales, Janaina Fernandes, Manuella Lanzetti, Samuel S Valença, Paolo Pelosi, Cerli R Gattass, Patricia R M Rocco
• Journal: Respiratory physiology & neurobiology (Respir Physiol Neurobiol) Vol. 179 Issue 2-3 Pg. 129-36 (Dec 15 2011) ISSN: 1878-1519 [Electronic] Netherlands
• PMID: 21801858 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Chemokines
• Reactive Oxygen Species
• Oleanolic Acid

Topics

• Acute Lung Injury (immunology, pathology)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Bronchoalveolar Lavage Fluid (chemistry)
• Chemokines (analysis, biosynthesis)
• Disease Models, Animal
• Inflammation (immunology, pathology)
• Lung (drug effects, immunology, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Oleanolic Acid (pharmacology)
• Oxidative Stress (drug effects, immunology)
• Reactive Oxygen Species (analysis, immunology)
• Respiratory Mechanics (drug effects, immunology)