Abstract | BACKGROUND: Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. However, unlike CD8 T cells, CD4 T cells - especially self-reactive cells - are challenging to detect with major histocompatibility complex (MHC) class II tetramers because of low frequencies and low affinities of their T cell receptors to MHC- peptide complexes. Here, we report the use of fluorescent multimers, designated MHC dextramers that contain a large number of peptide-MHC complexes per reagent. RESULTS: CONCLUSIONS: The data suggest that MHC dextramers are useful tools to track the generation and functionalities of self-reactive CD4 cells in various experimental systems.
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Authors | Chandirasegaran Massilamany, Bijaya Upadhyaya, Arunakumar Gangaplara, Charles Kuszynski, Jay Reddy |
Journal | BMC immunology
(BMC Immunol)
Vol. 12
Pg. 40
(Jul 18 2011)
ISSN: 1471-2172 [Electronic] England |
PMID | 21767394
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantigens
- Epitopes
- Histocompatibility Antigens Class II
- Peptides
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Topics |
- Animals
- Autoantigens
(immunology)
- CD4-Positive T-Lymphocytes
(immunology)
- Epitopes
(immunology)
- Female
- Histocompatibility Antigens Class II
(chemistry, immunology)
- Lymphocyte Activation
(immunology)
- Mice
- Mice, Inbred C57BL
- Peptides
(chemical synthesis, immunology, metabolism)
- Sensitivity and Specificity
- Staining and Labeling
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