Validity and reliability of perinatal biomarkers of adiposity after storage as dried blood spots on paper.

Abstract

OBJECTIVE:

To validate use of chip-based immunoaffinity capillary electrophoresis on dried blood spot samples (DBSS) to measure obesity-related hormones.

METHODS:

Chip-based immunoaffinity capillary electrophoresis was used to measure adiponectin, leptin and insulin in capillary serum and DBSS in pregnant women and infant heelstick at birth and six weeks. Concordance of measurements was determined with Pearson's correlation and Bland-Altman plots.

RESULTS:

We report high concordance between results obtained from serum and DBSS.

CONCLUSIONS:

Ease of sample collection and storage makes DBSS an optimal method for use in studies involving neonates and young children, as well as studies conducted in areas where freezer storage is not available.

• Authors: Nicole L Mihalopoulos, Terry M Phillips, Hillarie Slater, J Anne Thomson, Michael W Varner, M Nazeem Nanjee, Laurie J Moyer-Mileur
• Journal: American journal of human biology : the official journal of the Human Biology Council (Am J Hum Biol) 2011 Sep-Oct Vol. 23 Issue 5 Pg. 717-9 ISSN: 1520-6300 [Electronic] United States
• PMID: 21735507 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
• Copyright: Copyright © 2011 Wiley-Liss, Inc.

Chemical References

• Adiponectin
• Biomarkers
• Insulin
• Leptin

Topics

• Adiponectin (blood)
• Adiposity
• Biomarkers (blood)
• Capillaries (chemistry)
• Dried Blood Spot Testing (instrumentation, methods)
• Electrophoresis, Microchip (instrumentation, methods)
• Female
• Humans
• Infant
• Infant, Newborn
• Insulin (blood)
• Leptin (blood)
• Obesity (diagnosis)
• Pregnancy
• Reproducibility of Results
• Sensitivity and Specificity