Abstract |
Continuous i.v. infusion of a nonlethal dose of Escherichia coli endotoxin induced an early (3-h) accumulation of neutrophils in the rat liver followed by a later (30-h) greater extravasation of mononuclear phagocytes (MNP). These inflammatory cells, recovered together by centrifugal elutriation, were analyzed for their potential capacity to metabolize [1-14C]-AA. Ca2+ ionophore A23187 (5 microM) stimulated the release of [1-14C]-AA from PC and PI both in cells from saline- and ET-infused rats, the latter showing a higher capacity to further metabolize AA to eicosanoids. LTB4 and 5-HETE were the major metabolites accumulated in cells from rats infused with ET for 3 h, while PGD2 played the main role in cells from saline-infused rats. This could reflect [1-14C]-AA metabolism by PMNP and Kupffer cells, respectively. By 30 h of ET-infusion, a shift from PGD2 to PGE2 release was observed. These results suggest that eicosanoids released by nonparenchymal cells (i.e., Kupffer and endothelial cells) and PMNP in the liver of ET-infused rats may alter the normal intercellular information flow between parenchymal and nonparenchymal cells, contributing to the severe impairment in liver function and metabolism during endotoxicosis and sepsis.
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Authors | E B Rodriguez de Turco, J A Spitzer |
Journal | Journal of leukocyte biology
(J Leukoc Biol)
Vol. 48
Issue 6
Pg. 488-94
(Dec 1990)
ISSN: 0741-5400 [Print] England |
PMID | 2172432
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Arachidonic Acids
- Eicosanoids
- Endotoxins
- Hydroxyeicosatetraenoic Acids
- Leukotriene B4
- Arachidonic Acid
- Calcimycin
- 5-hydroxy-6,8,11,14-eicosatetraenoic acid
- Dinoprostone
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Topics |
- Animals
- Arachidonic Acid
- Arachidonic Acids
(metabolism)
- Calcimycin
(pharmacology)
- Dinoprostone
(biosynthesis)
- Eicosanoids
(biosynthesis)
- Endotoxins
(toxicity)
- Hydroxyeicosatetraenoic Acids
(biosynthesis)
- Leukotriene B4
(biosynthesis)
- Liver
(metabolism, pathology)
- Male
- Neutrophils
(pathology)
- Rats
- Rats, Inbred Strains
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