Abstract |
A cathepsin B-like cysteine protease belonging to family C1 is abundantly expressed in the transcriptome and proteome of the carcinogenic liver fluke of humans, Opisthorchis viverrini. This enzyme is present in excretory/secretory (ES) products released by parasites cultured in vitro. This study evaluated the performance of recombinant O. viverrini cathepsin B1 (rOv-CB-1) as an antigen for immunodiagnosis of opisthorchiasis. The full length Ov-CB-1 cDNA was cloned and recombinant protein was produced in catalytically active form in Pichia pastoris. The recombinant Ov-CB-1 (rOv-CB-1) was affinity purified via nickel-NTA chromatography and tested in enzyme-linked immunosorbent assays (ELISA) with human sera from an opisthorchiasis endemic area. Sera from egg-positive O. viverrini infections produced a strong IgG antibody response to rOv-CB-1 both in ELISA and immunoblot analysis. The sensitivity and specificity of the ELISA test was 67% and 81%, respectively. These findings support the feasibility of using recombinant Ov-CB-1 in ELISA for the serodiagnosis of human opisthorchiasis.
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Authors | Jittiyawadee Sripa, Paul J Brindley, Banchob Sripa, Alex Loukas, Sasithorn Kaewkes, Thewarach Laha |
Journal | Parasitology international
(Parasitol Int)
Vol. 61
Issue 1
Pg. 191-5
(Mar 2012)
ISSN: 1873-0329 [Electronic] Netherlands |
PMID | 21704728
(Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Antibodies, Helminth
- Antigens, Helminth
- Helminth Proteins
- Recombinant Proteins
- Cathepsin B
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Topics |
- Animals
- Antibodies, Helminth
(blood)
- Antigens, Helminth
(blood, genetics, metabolism)
- Cathepsin B
(genetics, immunology, metabolism)
- Chromatography, Affinity
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Helminth Proteins
(genetics, immunology, metabolism)
- Humans
- Immunoblotting
- Male
- Opisthorchiasis
(diagnosis, immunology, parasitology)
- Opisthorchis
(enzymology, genetics, growth & development, immunology)
- Pichia
- Recombinant Proteins
(genetics, immunology, metabolism)
- Sensitivity and Specificity
- Serologic Tests
(methods)
- Thailand
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