Abstract | BACKGROUND: METHODS: A panel of 15 HNSCC cell lines was assayed for glucose and glutamine dependence and sensitivity to metabolic inhibitors. In addition, broad-spectrum metabolomic analysis using mass spectrometry/liquid chromatography was combined with individual measurements of reducing potential, adenosine triphosphate, and lactate production to characterize cellular metabolic phenotypes. RESULTS:
HNSCC energy and reducing potential levels closely mirrored extracellular glucose concentrations. Glucose starvation induced cell death despite the activation of secondary energetic pathways. Conversely, glutamine was not required for HNSCC survival and did not serve as a significant source of energy. 2-deoxyglucose (2-DG) and its fluorinated derivative decreased glycolytic and Krebs cycle activity, cellular energy, and reducing potential and inhibited HNSCC cell proliferation. 2-DG effects were potentiated by the addition of metformin, but not by inhibitors of the pentose phosphate pathway or glutaminolysis. Despite dependence on glucose catabolism, the authors identified a subset of cell lines with relative resistance to starvation. Exploration of 1 such cell line (HN30) suggested that the presence of wild-type p53 can partially protect tumor cells from glucose starvation. CONCLUSIONS:
HNSCC tumor cells are dependent on glucose, not glutamine, for energy production and survival, providing a rationale for treatment strategies that target glucose catabolism. However, antimetabolic strategies may need to be tailored to the tumor background, more specifically, p53 status.
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Authors | Vlad C Sandulache, Thomas J Ow, Curtis R Pickering, Mitchell J Frederick, Ge Zhou, Izabela Fokt, Melinda Davis-Malesevich, Waldemar Priebe, Jeffrey N Myers |
Journal | Cancer
(Cancer)
Vol. 117
Issue 13
Pg. 2926-38
(Jul 01 2011)
ISSN: 1097-0142 [Electronic] United States |
PMID | 21692052
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 American Cancer Society. |
Chemical References |
- Tumor Suppressor Protein p53
- Glutamine
- Lactic Acid
- Adenosine Triphosphate
- Metformin
- Deoxyglucose
- Glucose
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Topics |
- Adenosine Triphosphate
(metabolism)
- Carcinoma
(metabolism)
- Carcinoma, Squamous Cell
- Cell Cycle
- Cell Line, Tumor
- Cell Proliferation
- Chromatography, Liquid
- Deoxyglucose
(pharmacology)
- Disease Progression
- Genes, p53
- Glucose
(metabolism)
- Glutamine
(metabolism)
- Head and Neck Neoplasms
(metabolism)
- Humans
- Lactic Acid
(metabolism)
- Mass Spectrometry
- Metabolomics
- Metformin
(pharmacology)
- Neoplasms, Squamous Cell
(metabolism)
- Squamous Cell Carcinoma of Head and Neck
- Treatment Outcome
- Tumor Suppressor Protein p53
(metabolism)
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