Abstract | BACKGROUND: AIMS: METHODS: The readouts were clinical outcomes, CAP-18 expression in mucosa of colon, rectum, lung and trachea (immunohistochemistry and real-time PCR) and release of the CAP-18 peptide/ protein in stool (Western blot). PRINCIPAL FINDINGS: Significant downregulation of CAP-18 expression in the epithelia of rectum and colon, the site of Shigella infection was confirmed. Interestingly, reduced expression of CAP-18 was also noticed in the epithelia of lung and trachea, indicating a systemic effect of the infection. This suggests a causative link to acute respiratory infections during shigellosis. Oral treatment with PB resulted in reduced clinical illness and upregulation of CAP-18 in the epithelium of rectum. Both PB and NaB counteracted the downregulation of CAP-18 in lung epithelium. The drug effect is suggested to be systemic as intravenous administration of NaB could also upregulate CAP-18 in the epithelia of lung, rectum and colon. CONCLUSION: Our results suggest that PB has treatment potential in human shigellosis. Enhancement of CAP-18 in the mucosal epithelia of the respiratory tract by PB or NaB is a novel discovery. This could mediate protection from secondary respiratory infections that frequently are the lethal causes in dysentery.
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Authors | Protim Sarker, Sultan Ahmed, Snigdha Tiash, Rokeya Sultana Rekha, Roger Stromberg, Jan Andersson, Peter Bergman, Gudmundur H Gudmundsson, Birgitta Agerberth, Rubhana Raqib |
Journal | PloS one
(PLoS One)
Vol. 6
Issue 6
Pg. e20637
( 2011)
ISSN: 1932-6203 [Electronic] United States |
PMID | 21673991
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimicrobial Cationic Peptides
- Phenylbutyrates
- RNA, Messenger
- Cathelicidins
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Topics |
- Administration, Oral
- Amino Acid Sequence
- Animals
- Antimicrobial Cationic Peptides
(chemistry, genetics, metabolism)
- Bacterial Load
(drug effects)
- Down-Regulation
(drug effects)
- Dysentery, Bacillary
(drug therapy)
- Feces
(microbiology)
- Intestinal Mucosa
(drug effects, metabolism, microbiology)
- Intestine, Large
(drug effects, metabolism, microbiology)
- Lung
(drug effects, metabolism, microbiology)
- Molecular Sequence Data
- Phenylbutyrates
(adverse effects, pharmacology, therapeutic use)
- RNA, Messenger
(genetics, metabolism)
- Rabbits
- Shigella
(drug effects, pathogenicity)
- Trachea
(drug effects, metabolism, microbiology)
- Cathelicidins
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