Identification of c-myc-dependent proteins in the medulloblastoma cell line D425Med.

Abstract	High c-myc levels are linked to poor prognosis in medulloblastoma (MB), and it was the aim of the current study to search for c-myc-dependent proteins in the MB cell line D425Med. For this purpose D425Med cells and cells with knocked-down c-myc (by siRNA) were analysed by a gel-based differential proteomics study using mass spectrometry. Heterogeneous nuclear ribonucleoproteins C1/C2, heterogeneous nuclear ribonucleoprotein A/B, stathmin, endoplasmic reticulum protein ERp29 precursor and guanidinoacetate N-methyltransferase were c-myc dependently expressed. Signalling, the protein machinery, metabolism and endoplasmic reticulum function may be affected and these results enable studying tumour tissue for these proteins as potential dignity markers or pharmacological targets.
Authors	Amedeo A Azizi, Lin Li, Thomas Ströbel, Wei-Qiang Chen, Irene Slavc, Gert Lubec
Journal	Amino acids (Amino Acids) Vol. 42 Issue 6 Pg. 2149-63 (Jun 2012) ISSN: 1438-2199 [Electronic] Austria
PMID	21667264 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Biomarkers, Tumor ERP29 protein, human Heat-Shock Proteins Heterogeneous-Nuclear Ribonucleoproteins Protein Precursors Proto-Oncogene Proteins c-myc RNA, Small Interfering STMN1 protein, human Stathmin Guanidinoacetate N-Methyltransferase
Topics	Biomarkers, Tumor (genetics, metabolism) Cell Line, Tumor Endoplasmic Reticulum (genetics, metabolism) Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Guanidinoacetate N-Methyltransferase (genetics, metabolism) Heat-Shock Proteins (genetics, metabolism) Heterogeneous-Nuclear Ribonucleoproteins (genetics, metabolism) Humans Medulloblastoma (genetics, metabolism, pathology) Protein Precursors (genetics, metabolism) Proto-Oncogene Proteins c-myc (genetics, metabolism) RNA, Small Interfering (genetics) Signal Transduction (genetics) Stathmin (genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!