Effect of aspirin on the expression of hepatocyte NF-κB and serum TNF-α in streptozotocin-induced type 2 diabetic rats.

Abstract

Aspirin is a kind of anti-inflammatory drug and may be used to reverse hyperglycemia, hyperinsulinemia, and dyslipidemia by improving insulin resistance. We hypothesized that aspirin improves insulin resistance in type 2 diabetes by inhibiting hepatic nuclear factor kappa-β (NF-κB) activation and serum tumor necrosis factor-α (TNF-α). Adult male Wistar rats were randomly divided into four groups: control, untreated diabetic, diabetic treated with metformin (100 mg/kg/day), and diabetic treated with aspirin (120 mg/kg/day). Diabetes was induced by high-fat feeding and a low dose of streptozotocin (30 mg/kg). After treatment, plasma glucose, insulin, lipids, free fatty acids (FFAs) concentrations and serum TNF-α were determined. The expression of NF-κB in hepatocytes was analyzed by immunohistochemistry and western blot. The results showed administration of aspirin caused no significant lowering in fasting glucose level but significant reduction of hepatic NF-κB expression and serum TNF-α level with improved insulin resistance compared to the diabetic group. The relevant analysis showed positive correlation between the expression of homeostasis model assessment-insulin resistance (HOMA-IR) and NF-κB (r = 0.799, P < 0.01); HOMA-IR and serum TNF-α (r = 0.790, P < 0.01). It is concluded that aspirin improves insulin resistance by inhibiting hepatic NF-κB activation and TNF-α level in streptozotocin-induced type 2 diabetic rats.

• Authors: Xiaodong Sun, Fang Han, Junling Yi, Lina Han, Ben Wang
• Journal: Journal of Korean medical science (J Korean Med Sci) Vol. 26 Issue 6 Pg. 765-70 (Jun 2011) ISSN: 1598-6357 [Electronic] Korea (South)
• PMID: 21655062 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Blood Glucose
• Fatty Acids, Nonesterified
• Hypoglycemic Agents
• Insulin
• NF-kappa B
• Tumor Necrosis Factor-alpha
• Metformin
• Aspirin

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Aspirin (pharmacology)
• Blood Glucose (analysis)
• Diabetes Mellitus, Experimental (blood, chemically induced, metabolism)
• Fatty Acids, Nonesterified (blood)
• Hypoglycemic Agents (pharmacology)
• Insulin (blood)
• Insulin Resistance
• Liver (metabolism)
• Male
• Metformin (therapeutic use)
• NF-kappa B (metabolism)
• Rats
• Rats, Wistar
• Tumor Necrosis Factor-alpha (blood)