Reversible thrombin detection by aptamer functionalized STING sensors.

Abstract

Signal Transduction by Ion NanoGating (STING) is a label-free technology based on functionalized quartz nanopipettes. The nanopipette pore can be decorated with a variety of recognition elements and the molecular interaction is transduced via a simple electrochemical system. A STING sensor can be easily and reproducibly fabricated and tailored at the bench starting from inexpensive quartz capillaries. The analytical application of this new biosensing platform, however, was limited due to the difficult correlation between the measured ionic current and the analyte concentration in solution. Here we show that STING sensors functionalized with aptamers allow the quantitative detection of thrombin. The binding of thrombin generates a signal that can be directly correlated to its concentration in the bulk solution.

• Authors: Paolo Actis, Adam Rogers, Jeff Nivala, Boaz Vilozny, R Adam Seger, Olufisayo Jejelowo, Nader Pourmand
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 26 Issue 11 Pg. 4503-7 (Jul 15 2011) ISSN: 1873-4235 [Electronic] England
• PMID: 21636261 (Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Aptamers, Nucleotide
• Thrombin

Topics

• Aptamers, Nucleotide
• Biosensing Techniques (instrumentation, methods)
• Blood Chemical Analysis (instrumentation, methods)
• Electrochemical Techniques
• Equipment Design
• Humans
• Nanopores
• Nanotechnology
• Signal Transduction
• Thrombin (analysis)