Abstract | BACKGROUND: OBJECTIVES: To isolate MSCs in patients with psoriasis and to compare them with those obtained from atopic and healthy subjects, in order to analyse whether MSCs show some typical psoriatic profiles and to understand whether pathophysiological events leading to psoriasis start early at the stem cell level. METHODS: MSCs isolated from seven patients with psoriasis, seven patients with acute atopic dermatitis and seven healthy subjects were characterized by fluorescence-activated cell sorting analysis. VEGF and nitric oxide (NO) content was measured in conditioned medium, the expression of VEGF and iNOS was analysed by immunohistochemistry, and the total oxyradical scavenging capacity towards peroxynitrite was tested. RESULTS:
VEGF content was highest in the medium conditioned by psoriatic perilesional MSCs, whereas NO concentration was maximally increased in medium conditioned by MSCs isolated from lesional psoriatic skin. The ability to neutralize the oxidizing effects of peroxynitrite was lower for MSCs isolated from lesional psoriatic skin compared with other MSCs, except for MSCs of lesional atopic skin. CONCLUSIONS: The microenvironment in psoriasis differs from those of atopic dermatitis and healthy skin; it could induce resident MSCs to produce angiogenic and proinflammatory mediators which lead to a reduction in the antioxidant capacity of these cells, contributing to the development of skin lesions in psoriasis.
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Authors | M Orciani, A Campanati, E Salvolini, G Lucarini, G Di Benedetto, A Offidani, R Di Primio |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 165
Issue 3
Pg. 585-92
(Sep 2011)
ISSN: 1365-2133 [Electronic] England |
PMID | 21623755
(Publication Type: Journal Article)
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Copyright | © 2011 The Authors. BJD © 2011 British Association of Dermatologists. |
Chemical References |
- Vascular Endothelial Growth Factor A
- NOS2 protein, human
- Nitric Oxide Synthase Type II
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Topics |
- Adult
- Aged
- Biopsy
- Case-Control Studies
- Cells, Cultured
- Chronic Disease
- Female
- Humans
- Immunohistochemistry
- Male
- Mesenchymal Stem Cells
- Middle Aged
- Nitric Oxide Synthase Type II
(metabolism)
- Psoriasis
(pathology)
- Skin
(pathology)
- Vascular Endothelial Growth Factor A
(metabolism)
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