Abstract |
The flap endonuclease-1 (FEN-1) gene is involved in DNA replication and repair, and it maintains genomic stability as well as the accuracy of DNA replication under normal growth conditions. However, FEN-1 also plays an important role in apoptosis and cancer development. We cloned the BmFEN-1 gene from Bombyx mori, which was 1343 bp in length and possessed an 1143 bp ORF (123-1266). It consists of seven introns and eight exons that encode a protein with 380 amino acids that has the typical XPG domain. The N-terminal motif is located at amino acids 95-105, and the proliferating cell nuclear antigen interaction motif is located at amino acids 337-344. RNA interference-mediated reduction of BmFEN-1 expression induced cell cycle arrest in S phase in BmE-SWU1 cells. These results suggest that BmFEN-1 can inhibit apoptosis and promote cell proliferation.
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Authors | Min-Hui Pan, Juan Du, Jin-Ye Zhang, Mao-Hua Huang, Ting Li, Hong-Juan Cui, Cheng Lu |
Journal | DNA and cell biology
(DNA Cell Biol)
Vol. 30
Issue 10
Pg. 763-70
(Oct 2011)
ISSN: 1557-7430 [Electronic] United States |
PMID | 21612397
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Insect Proteins
- Flap Endonucleases
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Topics |
- Animals
- Apoptosis
- Base Sequence
- Bombyx
(genetics, metabolism)
- Cell Cycle
(genetics)
- Cell Proliferation
- Cell Survival
- Cells, Cultured
- Cloning, Molecular
(methods)
- Databases, Genetic
- Exons
- Flap Endonucleases
(chemistry, genetics, metabolism)
- Insect Proteins
(chemistry, genetics, metabolism)
- Introns
- Larva
(genetics, metabolism)
- Molecular Sequence Data
- Open Reading Frames
- Phylogeny
- Protein Structure, Tertiary
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Transfection
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