Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay.

Abstract

A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

• Authors: Owen Kavanagh, Mary K Estes, Amanda Reeck, Ravikiran M Raju, Antone R Opekun, Mark A Gilger, David Y Graham, Robert L Atmar
• Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 18 Issue 7 Pg. 1187-90 (Jul 2011) ISSN: 1556-679X [Electronic] United States
• PMID: 21593238 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antibodies, Viral
• Immunoglobulin A
• Immunoglobulin G

Topics

• Antibodies, Viral (analysis, blood)
• Caliciviridae Infections (diagnosis)
• Enzyme-Linked Immunosorbent Assay
• Fluoroimmunoassay (methods)
• Humans
• Immunoglobulin A (analysis)
• Immunoglobulin G (analysis)
• Norwalk virus (immunology)
• Serologic Tests
• Time Factors