Overexpression of prothymosin alpha predicts poor disease outcome in head and neck cancer.

Abstract

BACKGROUND:

In our recent study, tissue proteomic analysis of oral pre-malignant lesions (OPLs) and normal oral mucosa led to the identification of a panel of biomarkers, including prothymosin alpha (PTMA), to distinguish OPLs from histologically normal oral tissues. This study aimed to determine the clinical significance of PTMA overexpression in oral squamous cell hyperplasia, dysplasia and head and neck squamous cell carcinoma (HNSCC).

METHODOLOGY:

Immunohistochemistry of PTMA protein was performed in HNSCCs (n = 100), squamous cell hyperplasia (n = 116), dysplasia (n = 50) and histologically normal oral tissues (n = 100). Statistical analysis was carried out to determine the association of PTMA overexpression with clinicopathological parameters and disease prognosis over 7 years for HNSCC patients.

RESULTS:

Our immunohistochemical analysis demonstrated significant overexpression of nuclear PTMA in squamous cell hyperplasia (63.8%), dysplasia (50%) and HNSCC (61%) in comparison with oral normal mucosa (p(trend)<0.001). Chi-square analysis showed significant association of nuclear PTMA with advanced tumor stages (III+IV). Kaplan Meier survival analysis indicated reduced disease free survival (DFS) in HNSCC patients (p<0.001; median survival 11 months). Notably, Cox-multivariate analysis revealed nuclear PTMA as an independent predictor of poor prognosis of HNSCC patients (p<0.001, Hazard's ratio, HR = 5.2, 95% CI = 2.3-11.8) in comparison with the histological grade, T-stage, nodal status and tumor stage.

CONCLUSIONS:

Nuclear PTMA may serve as prognostic marker in HNSCC to determine the subset of patients that are likely to show recurrence of the disease.

• Authors: Satyendra Chandra Tripathi, Ajay Matta, Jatinder Kaur, Jorg Grigull, Shyam Singh Chauhan, Alok Thakar, Nootan Kumar Shukla, Ritu Duggal, Ajoy Roy Choudhary, Siddhartha Dattagupta, Mehar Chand Sharma, Ranju Ralhan, K W Michael Siu
• Journal: PloS one (PLoS One) Vol. 6 Issue 5 Pg. e19213 (May 05 2011) ISSN: 1932-6203 [Electronic] United States
• PMID: 21573209 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protein Precursors
• prothymosin alpha
• Thymosin

Topics

• Adult
• Aged
• Female
• Head and Neck Neoplasms (metabolism, pathology)
• Humans
• Immunoblotting
• Immunohistochemistry
• Male
• Middle Aged
• Protein Precursors (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Thymosin (analogs & derivatives, metabolism)
• Young Adult