Abstract | PURPOSE: METHODS: This study was a prospective, randomized, controlled animal study performed using adult male Sprague-Dawley rats. Sepsis was induced by cecal ligation and perforation. At 18 h, surviving animals were randomized to spontaneous breathing or two strategies of MV for 4 h: low tidal volume (V (T)) (6 ml/kg) plus 10 cmH2O of positive end-expiratory pressure (PEEP) versus high (20 ml/kg) tidal volume (V (T)) with zero PEEP. Histological evaluation, measurements of WNT5A, total β- catenin, and matrix metalloproteinase-7 (MMP7) protein levels by Western blot, and their immunohistochemical localization in the lungs were analyzed. RESULTS:
Sepsis and high-V (T) MV caused lung inflammation and perivascular edema with cellular infiltrates and collagen deposition. Protein levels of WNT5A, β- catenin, and MMP7 in the lungs were increased in animals with sepsis-induced ALI. High-V (T) MV was associated with higher levels of WNT5A, β- catenin, and MMP7 protein levels (p < 0.001), compared to healthy control animals. By contrast, low-V (T) MV markedly reduced WNT5A, β- catenin, and MMP7 protein levels (p < 0.001). CONCLUSIONS:
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Authors | Jesús Villar, Nuria E Cabrera, Milena Casula, Francisco Valladares, Carlos Flores, Josefina López-Aguilar, Lluis Blanch, Haibo Zhang, Robert M Kacmarek, Arthur S Slutsky |
Journal | Intensive care medicine
(Intensive Care Med)
Vol. 37
Issue 7
Pg. 1201-9
(Jul 2011)
ISSN: 1432-1238 [Electronic] United States |
PMID | 21567117
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Wnt Proteins
- beta Catenin
- Matrix Metalloproteinase 7
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Topics |
- Animals
- Blotting, Western
- Disease Progression
- Immunohistochemistry
- Male
- Matrix Metalloproteinase 7
(metabolism)
- Prospective Studies
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Sepsis
(metabolism, physiopathology)
- Signal Transduction
- Ventilator-Induced Lung Injury
(metabolism, physiopathology)
- Ventilators, Mechanical
- Wnt Proteins
(metabolism)
- beta Catenin
(metabolism)
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