[The expression and relationship of CXCR4 and microvessel density in laryngeal squamous cell carcinoma].

Abstract

OBJECTIVE:

To investigate the expression of chemokine receptor 4 (CXCR4) in laryngeal squamous carcinoma and its correlation with clinicopathology character and the building of microvessel density (MVD). To evaluate its role in the carcinogenesis and progression in laryngeal squamous carcinoma.

METHOD:

The expression of CXCR4 in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 42 patients were examined by RT-PCR, immunohistochemical staining and Image-pro-plus software. The numbers of regeneration blood vessels in the laryngeal squamous carcinoma was counted by antibody against factor V associated antigen and immunohistochemical analysis.

RESULT:

The positive expression rate of CXCR4 in tumor samples was significantly higher than that in normal ones (P < 0.01). In tumor samples, the expression of CXCR4 were not associated with age, sex, tumor site and T stage (P > 0.05), while it were higher in tumors of grade III, IV than in grade I, II of pathology classification (P < 0. 01). The expression of CXCR4 were significantly higher in tumors with cervical lymph node metastasis than that in tumor without cervical lymph node metastasis (P < 0.01). The expression of CXCR4 protein and CXCR4 mRNA were at the same level. The expression level of CXCR4 in the laryngeal squamous carcinoma tissue was positively correlated with vascularization.

CONCLUSION:

The higher expression of CXCR4 may play a key role in the invasion and metastasis of laryngeal squamous carcinoma, and were correlated with micro-vascularization.

• Authors: Chuanming Zheng, Rongming Ge, Minghua Ge, Xicai Sun
• Journal: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery (Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 25 Issue 3 Pg. 123-6 (Feb 2011) ISSN: 2096-7993 [Print] China
• PMID: 21553525 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CXCR4 protein, human
• Receptors, CXCR4

Topics

• Adult
• Aged
• Aged, 80 and over
• Carcinoma, Squamous Cell (blood supply, metabolism, pathology)
• Female
• Humans
• Laryngeal Neoplasms (blood supply, metabolism, pathology)
• Lymphatic Metastasis
• Microvessels (pathology)
• Middle Aged
• Neoplasm Staging
• Neovascularization, Pathologic (pathology)
• Receptors, CXCR4 (metabolism)