We performed interphase in situ hybridization (ISH) for chromosomes 12 and X and now cytometric
DNA analysis on seven juvenile
granulosa cell tumors to verify this observation and correlate the results with clinicopathologic factors. Five cases were primary ovarian
tumors and two were metastatic lesions. Our results show that four
tumors exhibited polysomy 12 and four had
monosomy X; only two
tumors displayed concurrent aberrations of both chromosomes. Of the six
tumors with interpretable flow cytometric histograms three showed
DNA aneuploidy and three were
DNA diploid. All three
aneuploid tumors manifested polysomy 12. Of the three diploid
DNA neoplasms two showed
monosomy X and one displayed disomy for chromosomes 12 and X. No apparent correlation between numerical
chromosomal abnormalities and the
biological course was observed in this small cohort. Our results indicate that chromosomes 12 and X are frequently altered in these
neoplasms and thus could be targeted for further molecular studies in order to identify genetic aberrations which might be associated with JGCT
tumorigenesis.