Abnormal innervation patterns in the anorectum of ETU-induced fetal rats with anorectal malformations.

Abstract

To investigate whether anorectal malformations (ARMs) were associated with a global neuromuscular maldevelopment of the lower gastrointestinal (GI) tract and anorectum, the distribution of neuronal markers protein gene product (PGP9.5), nitric oxide synthases (NOs), neuromuscular junction markers (synaptophysin, SYP), interstitial cells of Cajal (ICC) marker (c-kit) within the terminal rectum were analyzed by immunohistochemistry and Western blot in rat embryos with ethylenethiourea (ETU) induced ARMs. From Gestational day16 (Gd16) to Gd21, neural crest-derived cells (NCC) migrated from the proximal gut into the terminal colon, colonising it along its entire length, gradually proliferated and differentiated to innervate the distal gut. From Gd19 to Gd21, significant gross-morphological differences of the anorectum of normal (n=90) and ARMs (n=90) embryos were found. Different myenteric plexus (MPs) development of the anorectum suggested that ARMs were associated with a global abnormal innervation patterns in the anorectum in gestational course and might have some postoperative effect.

• Authors: Weilin Wang, Huimin Jia, Hailan Zhang, Qingjiang Chen, Tao Zhang, Yuzuo Bai, Zhengwei Yuan
• Journal: Neuroscience letters (Neurosci Lett) Vol. 495 Issue 2 Pg. 88-92 (May 16 2011) ISSN: 1872-7972 [Electronic] Ireland
• PMID: 21440597 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011. Published by Elsevier Ireland Ltd.

Chemical References

• Biomarkers
• Synaptophysin
• Nitric Oxide Synthase
• Proto-Oncogene Proteins c-kit
• UCHL1 protein, rat
• Ubiquitin Thiolesterase

Topics

• Anal Canal (abnormalities, embryology, innervation)
• Animals
• Anorectal Malformations
• Anus, Imperforate (chemically induced, pathology, physiopathology)
• Biomarkers (metabolism)
• Enteric Nervous System (abnormalities, embryology, metabolism)
• Female
• Gestational Age
• Myenteric Plexus (abnormalities, embryology, metabolism)
• Neural Crest (cytology, metabolism)
• Neuromuscular Junction (metabolism)
• Neurons (metabolism)
• Nitric Oxide Synthase (metabolism)
• Proto-Oncogene Proteins c-kit (metabolism)
• Rats
• Rats, Wistar
• Rectum (abnormalities, embryology, innervation)
• Synaptophysin (metabolism)
• Ubiquitin Thiolesterase (metabolism)