Development of a recombinant CHO cell model for the investigation of CAR and DAF role during early steps of echovirus 6 infection.

Abstract

The early steps of echovirus 6 (E6) infection remain poorly understood and the only described receptor for haemagglutinating E6 strains is the decay accelerating factor (DAF). There is, however, accumulating evidence suggesting that E6 interaction with DAF is necessary but not sufficient for infection. In this report, we investigated the role of the coxsackie-adenovirus-receptor (CAR) as a potential DAF co-receptor during E6 infection. Using stably transfected Chinese Hamster Ovary (CHO) cells expressing CAR and DAF receptors, we found that DAF expression allowed attachment of both haemagglutinating and non-haemagglutinating E6 strains but was not sufficient for promoting E6 cell entry. Interestingly, the co-expression of DAF and CAR rendered 0.1-0.2% of cells permissive to some E6 strains' infection. Although our results did not show a major role of the CAR/DAF cooperation for E6 infection, it nevertheless indicated the use of CAR in the cell entry step of some minor E6 quasispecies. Moreover, the present report validates the use of recombinant CHO cells as valuable cellular model for the further characterisation of E6 receptors.

• Authors: Fanny Renois, Saw-See Hong, Richard Le Naour, Valérie Gafa, Déborah Talmud, Laurent Andréoletti, Nicolas Lévêque
• Journal: Virus research (Virus Res) Vol. 158 Issue 1-2 Pg. 46-54 (Jun 2011) ISSN: 1872-7492 [Electronic] Netherlands
• PMID: 21420451 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• CD55 Antigens
• CLMP protein, human
• Coxsackie and Adenovirus Receptor-Like Membrane Protein
• Receptors, Virus

Topics

• Animals
• CD55 Antigens (metabolism)
• CHO Cells
• Coxsackie and Adenovirus Receptor-Like Membrane Protein
• Cricetinae
• Cricetulus
• Echovirus 6, Human (physiology)
• Humans
• Receptors, Virus (metabolism)
• Virus Internalization