Abstract | OBJECTIVE: To identify the presence of orexin receptor 1 (OXR1) in obese rat pancreas and clarify its effect and mechanism on body weight and metabolic index in obese rats. METHODS: RESULTS: After feeding for 8 weeks, high-fat diet rats appear alimentary obesity body weight, serum glucose, insulin, triglyceride and cholesterol of high-diet rats were higher than those in the control group (P < 0.05). The expression of OX1R protein was located in the cytoplasm of pancreas Islet β-cells. The expression of OX1R protein in obese rat pancreas decreased around 16% versus the normal group (P < 0.05). After the obese rats were treated by OX1R-PS-ODNs for 72 hours, the expression of OX1R protein in pancreas declined from 82 ± 6 to 65 ± 4, reduced about 21% compare with control group (P < 0.01). CONCLUSION: The expression of OX1R protein is found in pancreas Islet β-cells. Hypothalamic orexin system can intervene the expression of OX1R protein in rat pancreatic tissue with alimentary obesity. And it may participate in the occurrence of obesity and the metabolic regulation of serum glucose and insulin.
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Authors | Lei Tian, Yu-Yan Zhao, Lu Wang, Dong-Jie Ma |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 91
Issue 6
Pg. 409-11
(Feb 15 2011)
ISSN: 0376-2491 [Print] China |
PMID | 21418915
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Glucose
- Hcrtr1 protein, rat
- Insulin
- Orexin Receptors
- Receptors, G-Protein-Coupled
- Receptors, Neuropeptide
- Triglycerides
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Topics |
- Animals
- Blood Glucose
(analysis)
- Diet
(adverse effects)
- Disease Models, Animal
- Insulin
(blood, metabolism)
- Insulin-Secreting Cells
(metabolism)
- Male
- Obesity
(etiology, metabolism)
- Orexin Receptors
- Pancreas
(metabolism)
- Rats
- Rats, Wistar
- Receptors, G-Protein-Coupled
(metabolism)
- Receptors, Neuropeptide
(metabolism)
- Triglycerides
(blood)
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