Abstract | BACKGROUND: METHODS: Seven SNPs in the dCK gene were sequenced in six human pancreatic cancer cell lines. The chemosensitivity of these six cell lines to gemcitabine were evaluated in vitro with a Cell Counting Kit-8 (CCK-8) test. Inhibition rates were used to express the chemosensitivity of pancreatic cancer cell lines to gemcitabine. RESULTS: The genotype of the A9846G SNP in the dCK gene was determined in six human pancreatic cancer cell lines. The cell lines BxPC-3 and T3M4 carried the A9846G SNP genotype AG, whereas cell lines AsPC-1, Mia PaCa2, SW1990 and SU86.86 carried the GG genotype. Cell lines with the AG genotype (BxPC-3 and T3M4) were more sensitive to gemcitabine compared with cell lines with the GG genotype (AsPC-1, Mia PaCa2, SW1990 and SU86.86) and significantly different inhibition rates were observed between cell lines carrying the AG and GG genotypes (P < 0.01). CONCLUSIONS:
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Authors | Shuang Si, Quan Liao, Yu-Pei Zhao, Ya Hu, Qiang Zhang, Li-Li You |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 124
Issue 3
Pg. 419-22
(Feb 2011)
ISSN: 2542-5641 [Electronic] China |
PMID | 21362344
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimetabolites, Antineoplastic
- Deoxycytidine
- Deoxycytidine Kinase
- Gemcitabine
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Topics |
- Antimetabolites, Antineoplastic
(pharmacology)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Deoxycytidine
(analogs & derivatives, pharmacology)
- Deoxycytidine Kinase
(genetics)
- Genotype
- Humans
- Pancreatic Neoplasms
(enzymology, genetics)
- Polymorphism, Single Nucleotide
(genetics)
- Gemcitabine
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