Abstract | BACKGROUND: METHODS: Human lung cancer cell lines A549, Ma44-3, FT821 and human mesothelioma cell lines MSTO-211H, NCI-H290, Y-MESO-14 were incubated with 0.03% 5-ALA for 4 h. After incubation, protoporphyrin IX ( PpIX) fluorescence was detected using a fluorescence microscope. Pleural carcinosis was induced in severe combined immunodeficiency disease mice using the previous cell lines to test the efficacy of PDD in vivo. The mice were sacrificed 4 h after oral administration of 400 mg/kg of 5-ALA. We counted the visible tumors under white light then fluorescence light. RESULTS: In vitro, clear red fluorescence was observed in all cell lines. The mean fluorescence intensity was stronger in A549 and FT821 cells than Ma44-3 cells (165.59±26.49, 157.62±18.93 vs. 104.01±17.58). Also, MSTO-211H and NCI-H290 cells had stronger fluorescence intensity than Y-MESO-14 cells (142.51±26.85, 165.16±12.91 vs. 92.31±8.69). In vivo, the tumor detection rate of fluorescence diagnosis was 1.1-4.5 times higher than that of white light. The mean number of metastases detected by the PDD was significantly higher than that of white light for FT821 (p=0.004), Ma44-3 (p=0.006) and Y-MESO-14 cell lines (p=0.005), but not for A549, NCI-H290 and MSTO-211H cell lines. Small lesions were detected by fluorescence diagnosis even though the lesions were invisible macroscopically under white light. CONCLUSION: Our results suggest the possibility of clinical application of fluorescence diagnosis with intrapleural malignant tumors.
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Authors | Abdellah Hamed Khalil Ali, Hiromitsu Takizawa, Kazuya Kondo, Hisashi Matsuoka, Hiroaki Toba, Yasushi Nakagawa, Koichiro Kenzaki, Shoji Sakiyama, Soji Kakiuchi, Yoshitaka Sekido, Saburo Sone, Akira Tangoku |
Journal | Lung cancer (Amsterdam, Netherlands)
(Lung Cancer)
Vol. 74
Issue 1
Pg. 48-54
(Oct 2011)
ISSN: 1872-8332 [Electronic] Ireland |
PMID | 21353719
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Photosensitizing Agents
- Protoporphyrins
- Aminolevulinic Acid
- protoporphyrin IX
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Topics |
- Aminolevulinic Acid
(administration & dosage, chemistry)
- Animals
- Carcinoma
(diagnosis, metabolism, pathology, physiopathology)
- Cell Line, Tumor
- Fluorescence
- Humans
- Lung Neoplasms
(diagnosis, metabolism, pathology, physiopathology)
- Mesothelioma
(diagnosis, metabolism, pathology, physiopathology)
- Mice
- Mice, SCID
- Microscopy, Fluorescence
- Neoplasm Metastasis
- Neoplasms, Experimental
- Photosensitizing Agents
(administration & dosage, chemistry)
- Pleural Neoplasms
(diagnosis, metabolism, pathology, physiopathology)
- Protoporphyrins
(chemistry, metabolism)
- Sensitivity and Specificity
- Tumor Burden
(drug effects)
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