Intracellular efficacy of tumor-targeting group I intron-based trans-splicing ribozyme.

Abstract

BACKGROUND:

Group I intron-based trans-splicing ribozyme, which can specifically reprogram human telomerase reverse transcriptase (hTERT) RNA, could be a useful tool for tumor-targeted gene therapy. In the present study, the therapeutic feasibility of this ribozyme was investigated by analyzing trans-splicing efficacy in vivo as well as in cells.

METHODS:

We assessed transgene activation, degree of ribozyme expression, targeted hTERT mRNA level, or the level of trans-splicing products in hTERT(+) cells or in human tumor nodules xenografted in animals after ribozyme administration.

RESULTS:

The activity and efficacy of the trans-splicing ribozyme in cells was dependent on the amount of endogenous hTERT mRNA and/or the accumulation of ribozyme RNA in cells. Intracellular activity of the ribozyme reached a plateau when no more targetable substrate mRNA was available or the ribozyme RNA level was fully saturated. In addition, the efficacy of ribozyme in xenografted tumor tissues was dependent on the dose of the delivered ribozyme-encoding adenoviral vector, indicating the potential of the ribozyme expression level as a determining factor for the in vivo efficacy of the trans-splicing ribozyme. On the basis of these results, we enhanced the intracellular ribozyme activity by increasing the ribozyme expression level transcriptionally and/or post-transcriptionally.

CONCLUSIONS:

We analyzed ribozyme efficacy and determined the most influential factors of its trans-splicing reaction in mammalian cell lines as well as in vivo. The present study could provide insights into the optimization of the trans-splicing ribozyme-based RNA replacement approach to cancer treatment.

• Authors: Byung-Su Kwon, Jin Sook Jeong, You Sub Won, Chang Ho Lee, Kyung-Sik Yoon, Min Hyung Jung, In-Hoo Kim, Seong-Wook Lee
• Journal: The journal of gene medicine (J Gene Med) Vol. 13 Issue 2 Pg. 89-100 (Feb 2011) ISSN: 1521-2254 [Electronic] England
• PMID: 21322101 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 John Wiley & Sons, Ltd.

Chemical References

• RNA, Catalytic
• TERT protein, human
• Telomerase

Topics

• Adenoviridae (genetics)
• Animals
• Cell Line, Tumor
• Gene Expression Regulation, Neoplastic
• Genetic Vectors (genetics)
• HEK293 Cells
• HT29 Cells
• Humans
• Intracellular Space (enzymology)
• Introns (genetics)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasms (enzymology, genetics, pathology)
• RNA, Catalytic (genetics, metabolism)
• Telomerase (metabolism)
• Transplantation, Heterologous